酶催化-荧光猝灭法测定药物中的姜黄素

DETERMINATION OF CURCUMIN IN MEDICAMENT BY ENZYME-CATALYZED FLUORESCENCE QUENCHING METHOD

酪氨酸在辣根过氧化物酶催化下被H2O2氧化为强荧光物质S，姜黄素对其荧光产生猝灭作用，据此建立了测定姜黄素的新方法。姜黄素浓度c在0．10～16．0μg/mL范围内与F0／F（F和F0分别为姜黄素存在和不存在时产物S的荧光强度）呈线性关系，线性回归方程c=5．4552F0／F-5．4860，线性相关系数r=0．9996，检出限为0．02μg/mL，测定结果的相对标准偏差为0．79％（n=10），加标回收率为94．7％～102％。研究了pH值、各物质的量、反应时间、干扰离子等对测定的影响。该法可用于药物中姜黄素含量的测定。

Because of catalysis of horseradish peroxidase, tyrosine reacted with H2O2 to form the product S that was a strong fluorescence substance. To the product S, the curcumin acted as a quencher. A new method for determining curcumin was established based on the principle of fluorescence quenching. A linear relationship was obtained between Fo/F and the concentration of curcumin in the range of 0.10 - 16.0 μg/mL. The linear regression equation of the calibration graph was c = 5. 455 2F0/F - 5. 486 0 （F0 was the relative fluorescence intensity of oxidation products, F was the relative fluorescence intensity of oxidation products in the presence of curcumin and c was the concentration of curcumin）, with the correlation coefficient of 0.9996. The detection limit was 0.02 μg/mL, the relative standard deviation was 0.79% （n = 11 ） , and recoveries were 94.7% - 102%. Optimizations of experimental variables were studied. Tbis method can be used for the determination of curcumin in medicament.