绿原酸完全抗原的制备及鉴定

Preparation and characterization of chlorogenic acid complete antigen

目的制备绿原酸完全抗原,对完全抗原进行鉴定。方法通过碳二亚胺(EDC)法把绿原酸与载体蛋白牛血清白蛋白和卵蛋白进行偶联制备,采用紫外扫描法对合成抗原进行了鉴定;用绿原酸完全抗原免疫豚鼠,设生理盐水对照组,绿原酸标准溶液对照组。间接酶联免疫法测定免疫豚鼠抗血清的效价。结果完全抗原偶联成功,测定偶联比20,符合动物免疫的要求。ELISA法检测豚鼠抗血清效价,完全抗原组豚鼠抗血清稀释1∶128为阳性,大于空白复孔A值2倍。结论成功制备绿原酸完全抗原。

Objective Characterizate the complete antigen of the chlorogenic acid. Methods The chlorogenic acid was coupled to BSA as an immunogen and to OVA as a coating antigen using the coupling agent EDC. HCL The immunogen and coating antigen were characterizated by the ultraviolet scanner, the complete antigen was uesd to immnune the cony pig, Detect the antiserum by ELISA. Results The coupling ratio of the complete antigens was 20. the quinza pig antiserums in 1 ： 128 in OD values were more higher than 2 times of the negative comparison. Conclusion The complete antigen was preparated successfully