利用杂质分布图对甲基苯丙胺样品进行同批鉴定研究

Study on the linked samples of methamphetamine identified by the impurity profile

目的通过甲基苯丙胺(MA)杂质分布图,比较和分类不同案件中的MA晶体,为MA样品进行同批次鉴定提供依据。方法每份MA样品取50mg溶于1ml缓冲液(pH 7.0,0.1mol/L磷酸盐缓冲液0.8ml,10%Na2CO3水溶液0.2ml),用乙酸乙酯0.5ml萃取(含有四个内标,C10、C15、C20、C28),用气相色谱仪-火焰离子检测器(GC-FID)分析,使用DB-5毛细管柱(30m×0.32mm i..d×1.0μm),选择17个特征峰对样品进行比较和分类,相对峰面积取对数值后用欧氏距离进行评价。结果对北京市公安局2006～2007年间缴获的8批MA样品进行分析,每批样品取6份,共48份样品进行实验,相同批次的样品成功地被分到同一组。结论本实验方法能很好地对MA样品进行同批次鉴定,可为毒品侦查工作提供有力的技术支持。

Objective To compare and classify the samples of MA crystals by using impurity profile to offer methods for identification of linked samples. Methods 50mg samples of MA were dissolved in 1 ml of buffer solution （pH 7.0, four parts of 0. lmol/L phosphate buffer, and one part of 10% Na2 CO3 ）, impurities were extracted with 0.5 ml of ethyl acetate containing four internal standards （n - decane, n-pentadecane, n-eicosane and n-octacosane） and analyzed by gas chromatography with a flame ionization detector on a DB-5 capillary column（30m×0.32mm i..d×1.0μm）. Seventeen characteristic peaks on chromatograms were selected for the comparison and classification of samples, and the data were evaluated by the Euclidean distance of the relative peak areas after logarithmic transformation. Results Eight different batches of MA （six samples from each batch） to be seized by the Beijing Public Security Bureau （BPSB） in 2006 - 2007 were analyzed. The results indicated that the samples from the same batch were successfully classified into one group. Conclusion The methods could be very useful for identifying the linked samples and could provide technique foundation for MA drug criminal inspection.