高效液相色谱法测定妇炎洁栓中大黄素与大黄酚的含量

Determination of both Emodin and Chrysophanol Levels in Fuyanjie Suppository by HPLC

目的建立妇炎洁栓中大黄素和大黄酚的含量测定方法。方法采用高效液相色谱法，流动相：甲醇-0．1％磷酸（78：22）；流速：0．9mL·min^-1；检测波长：254nm；柱温：40℃。结果大黄素和大黄酚分别在1．015～8．120μg·mL^-1和1．325—10．600μg·mL^-1范围内线性关系良好；大黄素的平均回收率为99．3％（RSD=1．20％），大黄酚的平均回收率为99．3％（RSD=1．35％）。结论该方法简便快速，结果准确、可靠，可用于该制剂的质量控制。

Objective To establish a method for the determination of emodin and chrysophanol levels in fuyanjie suppository. Methods The mobile phase in HPLC was consisted of methanol-0. 1% and phosphoric acid （78 ： 22 ） with 0.9 mL · min^-1 flow rate and 40 ℃ column temperature . The UV detecting wavelength was 254 nm. Results The linear relationship was strong under the concentration range of 1. 015 - 8. 120 μg·mL^-1 for emodin and range of 1. 325 10.600 μg·mL^-1 for chrysophanol. The average recoveries of emodin was 99.3% （ RSD = 1.20% ）, and chrysophanol was 99.3% （RSD = 1.35 % ）, respectively. Conclusion This method was proven to be simple and reliableand thus suitable for the quality control offuyanjie suppository.