高效毛细管电泳同时测定头孢噻肟与头孢羟氨苄

Simultaneous Determination of CFT and CFD by High Performance Capillary Electrophoresis

为建立同时测定头孢噻肟与头孢羟氨苄的高效毛细管电泳（HPCE）方法，考察了缓冲液离子浓度和pH，分离电压，运行温度等电泳参数。通过正交实验，确立了最佳电泳条件：缓冲液为40mmol／L硼砂，pH7．5（6mol／L HCL调节），紫外检测波长210nm，分离电压20kV，运行温度20℃。结果表明，两种药物在12min内得到完全分离。方法精密度（RSD）为0．09％～0．19％（迁移时间）和1．23％～1．98％（峰面积），回收率98．94％以上。

A high performance capillary electrophoresis method has been developed for the simultaneous separation and determination of Cefotaxime（CFT） and Cefadroxil（CFD） . The effects of several facters such as the pH and concentration ot running buffer, the separation voltage and the running temperature on HPCE were investigated. In optimum condition , the running buffer consisted of 40 mmol/L sodium tetraborate buffer（using 6 mol/L hydrochloric acid adjustsd to 7.5） ,the UV detection wavelength was 210nm, separation voltage 20 kV , running temperation at 20＂C . The results showed that CFT and CFD can be well separated within 12min. The RSD of migration time was 0.09-0.19%, and the peak area was 1.23%-1.98%. The average recovery was above 98.94%. The method is rapid,simple and accurate.