β-榄香烯对HepG2和HT-29细胞体外抗癌作用的实验研究

目的探讨β-榄香烯对肝癌和结肠癌的作用，为临床上利用β-榄香烯治疗肿瘤提供理论依据。方法体外培养HepG2肝癌细胞和HT-29结肠癌细胞，用生长曲线和MTT法观察β-榄香烯对两种细胞生长的影响。结果β-榄香烯组在培养的第6d、第7d、第8d能显著抑削HT-29细胞增殖；β-榄香烯在培养的第6d能显著抑制HepO2细胞增殖；在第2d、在第3d、在第4d，与对照组相比，β-榄香烯对HT-29和HepG2细胞增殖的抑制作用显著（P〈0．05）。结论β-榄香烯能明显抑制体外培养的HT-29和HepG2细胞生长。

Objective To explore the β-elemene on liver cancer and colon cancer in order to provide a theoretical basis for the clinical application.Methods Proliferation of HepG2 cell and HT-29 cell in vitro were observed with β -elemene by the means of growth curve modeling and MTT assay.Results β -elemene group in the sixth, seventh, eighth day, the can significantly Inhibit the HT-29 cells proliferation; the β -elemene group in the sixth day can significantly Inhibit the HepG2 cells proliferation; in the second day, third and fourth day, compared with the control group, the β -elemene group has significantly Inhibit the HT-29 and HepG2 cells proliferation. Conclusion β -elemene could inhibit the HT-29 and HepG2 cells growth in vitro.