摘要
利用紫杉幼枝顶芽作外植体,以MS为基本培养基,添加NAA与BAP不同浓度组合于培养基中,诱导顶芽萌发,形成丛生芽.结果表明:单独使用NAA时,最适浓度为1.0mg/L,产芽外植体为100%;同时使用NAA与BAP时,以NAA1.0mg/L加BAP0.1mg/L效果较好,产芽外植体为75%.
Shooting was induced from shoot tip bud on MS medium supplemented with different NAA and BAP concentration composition.The results indicate that the RES was 100% with 1.0mg/L NAA;the RES was 75% with NAA 1.0mg/L and BAP 0.1mg/L.