家兔激素性股骨头缺血坏死血管壁中差异表达基因的研究

Different expressions of genes from blood vessel walls in rabbits with steroid-treated osteonecrosis

目的运用基因芯片技术，筛选激素影响的血管壁中主要生长因子基因的差异表达情况，进一步探讨激素性股骨头缺血坏死的发病机理。方法中国大白兔25只，随机分为对照组（n=5），内毒素组（n=10）和模型组（n=10），利用大肠杆菌内毒素及甲泼尼龙诱导股骨头缺血坏死模型，分别取旋股内侧动、静脉，提取组织总RNA，与含有122个兔基因的寡核苷酸基因芯片杂交、洗涤、染色和扫描，分析检测数据。结果在检测的122个基因中，差异显著的基因动脉有10个，静脉有27个，生物学信息分析发现这些基因大部分与血管生成、炎症免疫、骨化相关。结论激素在导致股骨头缺血坏死的过程中降低了血管壁中主要促血管生长因子的转录，进而影响了其在血管壁中的生物活性。

Objective To explore the pathogenesis of steroid-induced osteonecrosis of the femoral head by identifying differentially expressed genes from blood vessel walls. Methods Twenty-five white rabbits were divided into 3 groups randomly： control, LPS and model ones. The model of steroid-induced avaseular necrosis of femoral head was established with LPS and methylprednisolone. Total RNAs were ex- tracted from the medial femoral circumflex arteries and veins, hybridized with the genes on the gene chip containing 122 genes, rinsed, stained and scanned. Data detected were analyzed. Results In the 122 genes, 73 genes differentially expressed in arteries and 76 in veins. Among them, 10 genes were significantly different in the artery and 27 genes in the vein. These genes were found to be correlated with angiogenesis, inflammation, immune and ossification. Conclusions In the process of steroid-induced avascular necrosis of femoral head, steroid down-regulates angiogenic growth factors and thus affects their biological activity in the blood vessel wall Down-regulation of angiogenic growth factors may be an important pathological basis for steroid-induced osteonecrosis.