神经生长因子对大鼠前脑缺血再灌注后细胞凋亡及Fas蛋白表达的影响

Effects of Nerve Growth Factor on the Expression of Fas Protein and Cell Apoptosis in the Hippocampus Following Cerebral Ischemia and Reperfusion in Rats

目的:研究神经生长因子(NGF)对大鼠前脑缺血再灌注后海马CA1区Fas蛋白表达和细胞凋亡的影响。方法:夹闭大鼠双侧颈总动脉造成前脑缺血,30 min后松夹再灌注,NGF组和生理盐水组于再灌注开始时分别肌肉注射NGF 30μg·mL^(-1)和生理盐水0.1 mL,应用免疫组化法和TUNEL法检测各组大鼠海马CA1区Fas蛋白表达和细胞凋亡。结果:再灌注后6和24 h,NGF组Fas蛋白平均吸光度值小于生理盐水组(P<0.001和P<0.05)。再灌注后48 h两组比较差异无统计学意义(P>0.5)。再灌注后6、24和48 h,NGF组TUNEL阳性细胞率均低于生理盐水组,差异有统计学意义(P<0.005)。结论:NGF可以减少大鼠脑缺血再灌注后海马CA1区Fas蛋白表达,抑制细胞凋亡,从而发挥其神经保护作用。

Aim： To study the effects of nerve growth factor on the expression of Fas protein and cell apoptosis in the hippocampus following cerebral ischemia and reperfusion in rats. Methods： The model of ischemic/reperfusion is established by clipping bilateral cervical arteries for 30 minutes and then removing the clips. The rats in nerve growth factor （NGF） and normal saline（NS） groups were given intramuscular injection of NGF and NS respectively at the beginning of reperfusion. Immunohistochemistry staining is used to detect the expression of Fas protein, and TUNEL to detect cell apoptosis in the region of hippocampus CA1. Results： The average optical density（AOD） of Fas protein is lower in NGF group than in NS group at 6h and 24h after reperfusion（P〈0.001, P〈0.05 respectively）. It shows no difference at 48 h （P〉0.5）. The percentage of apoptosis is lower in NGF group than in NS group at 6 h, 24 h and 48 h（P〈0.005）. Conclusion： NGF can downregulate the expression of Fas protein and reduce cell apoptosis in the hippocampus following cerebral ischemia and reperfusion in rats, to achieve the protective effects.