两种制备方法的桃花止泻冲剂的生物热动力学比较研究

Biothermo-kinetic comparative study on Taohua anti-diarrhea decoction prepared by two different ways

目的:对两种制备方法的桃花止泻冲剂进行生物热动力学比较研究,以评价其生物活性差异。方法:制备桃花止泻冲剂的粗粉制剂和微粉制剂,采用微量热法测定大肠杆菌在桃花止泻冲剂粗粉制剂和微粉制剂的水溶液作用下的生物热谱曲线,获得相应的生物热动力学参数,评价桃花止泻冲剂粗粉制剂和微粉制剂的生物活性差异。结果:随着桃花止泻冲剂的粗粉制剂和微粉制剂水溶液浓度的增大,大肠杆菌生长代谢的生长速率常数和最大产热功率值均减小,最高峰的出峰时间和生长抑制率均增大,表明细菌的生长受到抑制,且抑菌作用随着药物浓度的增大而增强。粗粉制剂的半抑制浓度值为35.9mg/ml,而微粉制剂的半抑制浓度值为31.4mg/ml。综合分析二者水溶液作用下大肠杆菌的生长代谢曲线和生物热动力学参数可知,桃花止泻冲剂微粉制剂的抑菌作用强于粗粉制剂。结论:微量热法可用于分析桃花止泻冲剂粗粉制剂和微粉制剂的生物活性差异,超微粉碎能增强桃花止泻冲剂的抑菌作用。

Objective：To compare the bioactivities of Taohua anti-diarrhea granula prepared by two different methods based on biothermo-kinetics study. Methods： The coarse and fine powder preparations of Taohua decoction were prepared. Microcalorimetry was used to obtain the metabolic curves and biothermo-kinetic parameters of Escherichia coli （E. coli） growth after treated by the two preparations to evaluate their bioactivities. Results：The values of growth rate constant k and maximum heat out power Pm declined with the concentration increase of the 2 preparations; meanwhile, the values of peak time of the highest peak tm and growth inhibitory ratio I increased with the concentration increase of the two preparations. The results showed that the growth of E. coli was inhibited and the inhibitory effect was strengthened with the concentration increase of the two preparations. The IC50was 35.9 mg/ml for the coarse powder preparation and 31.4 mg/ml for the fine powder preparation. Aggregate analysis of the metabolic curves and biothermo-kinetic parameters of E. coli indicated that the fine powder preparation of Taohua decoction had stronger inhibitory action against E. coli growth than the traditional coarse powder preparation. Conclusion：Microcalorimetry can be used to examine the bioactivity differences of the fine and coarse preparations of Taohua decoction. Superfine comminution may increase the inhibitory action of Taohua decoction.