大蒜油拮抗2，5-己二酮对大鼠神经组织的氧化损伤的效果

Effects of garlic oil on 2,5-HD induced lipid peroxidation damage in rat nerve tissues

目的探讨大蒜油对2，5-己二酮（2，5-hexanedione，2，5-HD）导致的大鼠神经组织氧化损伤的拮抗作用和对周围运动神经毒性的影响。方法Wistar雄性大鼠40只，随机分为正常对照组、模型组、大蒜油低、高剂量组，每组10只。模型组及大蒜油低、高剂量组分别给予2，5-HD300mg／kg腹腔注射，正常对照组给予生理盐水，5次/周，持续6周。大蒜油低、高剂量组提前1周分别给予40和80mg／kg大蒜油灌胃，持续至实验结束。测定后肢撑力指数和平衡指数等神经行为学指标，实验结束取脑、脊髓和坐骨神经分别测定丙二醛（MDA）、还原型谷胱甘肽（GSH）含量、总抗氧化能力（T—AOC）和抑制羟自由基能力。结果与第0周比较，后肢撑力指数第4周模型组升高44％，大蒜油低剂量组升高50％，大蒜油高剂量组升高49％，但3组间差异无统计学意义（P〉0．05）；第4周模型组平衡指数降低30％，大蒜油低剂量组降低45％，大蒜油高剂量组降低68％，与模型组相比，差异有统计学意义（P〈0．01）.大蒜油低、高剂量组大鼠在第4周即出现运动异常，较模型组大鼠提前1周；各组步态评分，模型组，大蒜油低、高剂量组均明显高于对照组，且大蒜油高剂量组高于模型组，差异均有统计学意义（P〈0．05）。在大脑、脊髓和坐骨神经中，与正常对照组相比，模型组大鼠MDA含量升高，抑制羟自由基能力降低，差异均有统计学意义（P〈0．05或P〈0．01）；与模型组比较，大蒜油低、高剂量组在各神经组织中MDA含量均明显降低，抑制羟自由基能力明显升高，差异均有统计学意义（P〈0．01）。结论大蒜油可拮抗2，5-HD所致的大鼠神经组织氧化损伤，但并未改善2，5-HD导致的周围运动神经损伤，提示氧化-抗氧化损伤不是2，5-HD中毒性神经病的主要机制。

Objective To investigate the effects of garlic oil（GO） against the peroxidation damage of rat nerve tissue and the peripheral motor neuropathy induced by 2,5-HD. Methods Male Wistar rats were divided into four groups, with 10 in each group. The model group,anti low and high doses of GO groups were administrated with 2,5-HD （ip, 300 mg/kg）, respectively; The control group was treated with sodium chloride, five times per week for six weeks. Pretreatment with GO garaged （40 mg/kg or 80 mg/kg） started one week before 2,5-HD treatment, and lasted to the end of the experiment. Neurobehavioral indexes were examined at the zero, second and fourth week. At the end of the experiment, the scores of the gait, and the concentration of MDA and GSH,the level of T-AOC and the ability of inhibition of· OH in cerebrum, spinal cord and sciatic nerve were examined. Results Compared with the zero week, except of the control group rats, the hind limb landing foot splays of three groups rats decreased by 44%, 50% and 49% at the fourth week, respectively without significant difference. The threshold value of balance in model,GO low and high doses groups rats decreased by 30%,45% and 68% at the fourth week, respectively, and lower than the control group rats（P〈0.01 ）. GO low and high doses groups rats showed the serious abnormity at the fourth week,before one week of the model group rats. The scores of gait of model, and GO low and high doses groups rats increased significantly compared with control group rats, and the GO high dose group rats were higher than model group rats（P〈0.05 ）. Increase of the concentration of MDA, and decrease of the level of the ability of inhibition of · OH were induced by 2,5-HD in cerebrum, spinal cord and sciatic nerve. The concentration of MDA increased, and the level of the ability of inhibition of · OH decreased （P〈0.05 or P〈0.01 ）, respectively. The results showed that the concentration of MDA decreased, and the level of the ability of inhibition of · OH induced by GO in cerebrum, spinal cord and sciatic nerve increased, the concentration of MDA of GO low doses group rats decreased, the level of the ability of inhibition of · OH increased, the concentration of MDA of GO high doses group rats decreased （P〈0.01 ）respectively, and the level of the ability of inhibition of·OH increased （P〈0.01） in nerve tissue. Conclusion GO has antagonist effect on the 2,5-HD induced peroxidation damage,but can not improve the function of the peripheral motor nerve, indicating that the lipid peroxidation does not play an important role in 2,5-HD neurotoxicity.