极快速减压后大鼠肺组织磷酯酶A2mRNA表达的改变

Changes of secretory phospholipase A2mRNA expression of lung tissue in rats after extremely fast decompression

目的研究极快速减压对大鼠肺组织ⅡA类磷酯酶A2（sPLA2）mRNA表达的影响。方法极快速减压处理后0～210min，间隔30min，分8个时间点对大鼠肺组织ⅡA类sPLA2mRNA表达水平进行连续检测，同时进行每个时间点的肺组织病理学特征和肺湿干重比检测。结果极快速减压处理后30min，大鼠肺组织ⅡA类sPLA2mRNA有一个表达高峰；大鼠肺组织病理损伤特征经历了一个由重至轻，再逐渐加重的反复变化过程；肺湿干重比有一个从重到轻的变化过程。结论大鼠经极快速减压处理出舱后210min内，肺损伤程度经历了一个由重到轻，然后再加重的过程；极快速减压能够导致大鼠肺组织ⅡA类sPLA2mRNA表达水平由低到高，然后再降低，其具体机制有待进一步研究。

Objective To explore the changes of secretory phospholipase A2 mRNA （sPLA2） expression in rats lung tissue after extremely fast decompression. Methods Rats treated with extremely fast decompression, were detected the expression of sPLA2 mRNA in rats lung 8 times from 0 to 210 rain,and the interval was 30 rain. At the same time, the pathological changes of rats lung and the lung wet/dry ratio were observed. Results Thirty min after the rats were treated the expression of sPLA2 mRNA in rats lung got an obvious peak. The pathological character of the lung injury experienced a repeated course from serious to slight and gradually aggravating again. The lung wet/dry mass ratio experienced a repeated course from large to small. Conclusions In 210 minutes after extremely fast decompression, the degree of rats lung injury experienced a course from serious to slight, then gradually aggravating again. The level of sPLA2 mRNA in a rats lung tissue treated with extremely fast decompression was from low to high, then stepped down again. This mechanism needs a further study.