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禽流感病毒NS1蛋白对细胞的影响 被引量:4

Effect of Influenza Virus NS1 Protein on Host Cell
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摘要 NS1蛋白为流感病毒非结构蛋白,只在病毒侵入宿主细胞后产生。目前NS1蛋白对细胞整体水平上的作用仍不清楚,为了解NS1蛋白在病毒感染细胞中的作用,构建了重组质粒pCMV-myc-NS1并将其转染A549细胞,利用双向电泳技术检测了受NS1蛋白调控的宿主蛋白,以期从蛋白质组水平上研究禽流感病毒与宿主细胞间的相互作用。同时,还检测了转染NS1对细胞增殖和细胞周期的影响。结果显示,NS1在细胞中的表达,能够明显引起宿主细胞代谢的变化,并通过阻滞细胞周期的正常进行而减缓细胞的增殖。 NS1 is a non-structural protein of the influenza A virus, which could only be expressed when cells are infected. The effect of NS1 protein on host cell is still not clear. To understand the role of NS1 protein in cell infection, recombinant plasmid pCMV-myc-NS 1 was constructed, and then transfected into A549 cells. Two-dimensional electrophoresis was employed to analyze proteins regulated by NS 1 that could reflect the interaction between influenza virus and host cells at the protein level. The influence of NS1 on cell proliferation and cell cycle was also studied. The result showed that not only could NS 1 remarkably affect metabolism but it could also slow down cell proliferation through blocking cell cycle.
出处 《生物工程学报》 CAS CSCD 北大核心 2008年第11期1912-1917,共6页 Chinese Journal of Biotechnology
基金 国家科技支撑计划项目(No.2006BAD06A01)资助~~
关键词 NS1蛋白 宿主细胞 双向电泳 细胞增殖 细胞周期 NS 1, host cell, two-dimensional electrophoresis, cell proliferation, cell cycle
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参考文献16

  • 1Qiu Y, Krug RM. The influenza virus NSI protein is a poly(A)-binding protein that inhibits nuclear export of mRNAs containing poly(A). J Virol, 1994, 68: 2425-2432.
  • 2Fortes P, Beloso A, Ortin J. Influenza virus NS1 protein inhibits pre-mRNA splicing and blocks mRNA nucleocytoplasmic transport. EMBO J, 1994, 13: 704-712.
  • 3Lu Y, Qian XY, Krug RM. The influenza virus NS1 protein: a novel inhibitor of pre-mRNA splicing. Genes Dev, 1994, 8: 1817-1828.
  • 4Li SD, Min JY, Krug RM, et al. Binding of the influenza A virus NS 1 protein to PKR mediates the inhibition of its activation by either PACT or double-stranded RNA. Virology, 2006, 349: ! 3-21.
  • 5Luna DL, Fortes SP, Beloso A. Influenza virus NS1 protein enhances the rate of translation initiation of viral mRNAs. J Virol, 1995, 69: 2427-2433.
  • 6Enami K, Sato TA, Nakada S, et al. Influenza virus NS1 protein stimulates translation of the M1 protein. J Virol, 1994, 68: 1432-1437.
  • 7Robert MK, Yuan WM, Diana L, et al. Intracellular warfare between human influenza viruses and human cells the roles of the viral NS1 protein. Virology, 2003, 309: 181-189.
  • 8Fernandez SA.The influenza virus NS1 protein: inhibitor of innate and adaptive immunity. Infect Disord Drug Targts, 2007, 7(4): 336-343.
  • 9Li Y, Anderson DH, Liu Q, et al. Mechanism of influenza A virus NS1 protein interaction with the p85β, but not the p85α, subunit of PI3K and upregulation of PI3K antivity. J Bio Chem, 2008, in Press.
  • 10Hale BG, Jackson D, Chen YH, et al. Influenza A virus NS1 protein binds p85β and activates phosphatidylinositol- 3-kinase signaling. Proc Natl Acad Sci USA, 2006, 103(38): 14194-14199.

二级参考文献10

  • 1Hopkins A L,Groom C R.The druggable genome.Nat Rev Drug Discov,2002,1(9):727 ~ 730.
  • 2Lehner I,Niehof M,Borlak J.An optimized method for the isolation and identification of membrane proteins.Electrophoresis,2003,24(11):1795 ~ 1808.
  • 3Jacobson B S,Stolz D B,Schnitzer J E.Identification of endothelial cell-surface proteins as targets for diagnosis and treatment of disease.Nat Med,1996,2(4):482~ 484.
  • 4Bordier C.Phase separation of integral membrane proteins in Triton X-114 solution.J Biol Chem,1981,256(4):1604 ~ 1607.
  • 5Zhang W,Zhou G,Zhao Y,White M A,Zhao Y.Affinity enrichment of plasma membrane for proteomics analysis.Electrophoresis,2003,24(16):2855 ~ 2863.
  • 6Jang J H,Hanash S.Profiling of the cell surface proteome.Proteomics,2003,3(10):1947 ~ 1954.
  • 7Zhao Y,Zhang W,Kho Y,Zhao Y.Proteomic analysis of integral plasma membrane proteins.Anal Chem,2004,76(7):1817~ 1823.
  • 8Rybak J N,Scheurer S B,Neri D,Elia G.Purification of biotinylated proteins on streptavidin resin:a protocol for quantitative elution.Proteomics,2004,4(8):2296~2299.
  • 9Berkelman T,Stenstedt T.2-D Electrophoresis:Using Immobilized pH Gradients,Principle and Methods.Amersham Pharmacia Biotech,Uppsala,Sweden,1998.
  • 10Liao X,Ying T Y,Huang P T,et al.A two-dimensional proteome map of Shigella flexneri.Electrophoresis,2003,24(16):2864 ~ 2882.

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