人源性骨髓间充质干细胞移植对脑缺血大鼠的行为影响

Effect of transplanted human bone marrow mesenchymal stem cells on behavioral changes of cerebral ischemic rats

目的研究骨髓间充质干细胞(BMSCs)脑内移植后对缺血性脑卒中大鼠的行为学影响。方法采集志愿者骨髓,分离、培养BMSCs;建立大鼠大脑中动脉栓塞缺血2h再灌注模型,分为治疗组12只和对照组12只,24h后脑内注射hBMSCs悬液15μL(2×1010L-1)和D-hank’s液15μL;移植后第4天开始进行NSS评分、黏贴物移除实验、转棒实验及Morris水迷宫实验的循环测试。结果治疗后第8天,水迷宫平均逃逸时间治疗组显著短于对照组(P<0.05),第29天趋同;平均游泳路程治疗组显著少于对照组,第32天趋同。第10天后,转棒实验(10r/min)平均潜伏期治疗组显著高于对照组。第13天,黏贴物移除实验平均潜伏期细胞治疗组显著快于对照组。NSS评分仅两个时间点有统计学差异。结论BMSCs脑内移植可以在卒中早期显著促进大鼠学习记忆、运动及感觉神经功能的恢复。

Objective Investigate the behaviors of cerebral ischemic rats after treatment with bone marrow mesenchymal stem cells（BMSCs）. Methods Bone marrow was collected and BMSCs were separated and cultivated. Twenty-four adult male Sprague-Dawley rats were performed transient （2 hours） middle cerebral arterial occlusion （MCAO） and then divided into treated group （n = 12） and control group （n = 12）. All rats received corresponding behavioral training before surgery, 15 μL hBMSCs （2 × 10^10cells/L） and D-hanks（ 15 μL） were injected into the brain cortex after 24 h of MCAO. Morris water maze test, NSS, Rotarod test and adhesive-removal test were performed serially and cyclically from the 4th day after transplantation. Results Since the 8th day after transplantation, the mean escape time and the mean swimming distance of treated group significantly are shorter than control＇s in Morris water maze test（P 〈0. 05）. Since the 10th day, the mean latency period of treated group is longer significantly than control＇ s in rotarod test with 10 r/min. Since the 13th day, the mean latency period of treated group is significantly shorter than control＇ s in adhensive-removal test. Only two time points are different in NSS between the two groups. Conclusion Transplantation of BMSCs improves the capability of learning and memory as well as the sensorimotor function recovery after stroke.