室旁核注射Apelin-13加重大鼠缺血-再灌注胃黏膜细胞凋亡

Apelin-13 microinjection into the paraventricular nucleus aggravates gastric mucosal cellular apoptosis induced by gastric ischemia-reperfusion in rats

目的研究大鼠室旁核(PVN)注射Apelin-13对胃缺血-再灌注(GI-R)的损伤作用及其细胞机制。方法PVN内微量注射Apelin-13,制备GI-R模型,用免疫组化方法检测胃黏膜细胞的凋亡、增殖及凋亡相关基因BCL-2和BAX的表达。结果(1)注射0.2、1.0和5.0μgApelin-13到PVN,GI-R损伤显著加重,且有剂量-效应依赖关系;(2)与GI-R组相比,注射Apelin-13能明显增加GI-R后胃黏膜细胞的凋亡,减低胃黏膜细胞的增殖,同时可以增加促凋亡因子BAX蛋白的表达,明显降低抗凋亡因子BCL-2蛋白的表达。结论室旁核内注射Apelin-13可促进胃黏膜细胞的凋亡、抑制其增殖,对GI-R损伤产生显著的加重作用。

Objective To observe effects of Apelin-13 microinjection into the hypothalamic paraventricular nucleus （PVN） on gastric ischemia-reperfusion（GI-R） injury in rats. Methods After Apelin-13 injection into PVN, the experimental model of GI-R was established by clamping the celiac artery for 30 min and then reperfused the artery for 1 h. We used immunohistochemistry to detect the gastric mucosal ceils apoptosis, proliferation and the expres- sion of BCL-2, BAX. Results （ 1 ） Apelin-13 microinjection into the PVN aggravated GI-R injury in an dose-de- pendent manner with dosages as 0. 2, 1.0 or 5.0 μg, respectively. （2） Compared with GI-R group, Apelin-13 microinjection into PVN markedly increased gastric mucosal cellular apoptosis, decreased the proliferation and promoted protein expression of BAX, but obviously inhibited the protein expression of BCL-2. Conclusion Apelin-13 mi- croinjection into the PVN may aggravate GI-R injury by promoting gastric mucosal cellular apoptosis and inhibiting proliferation.