摘要
目的用不同浓度的拓扑替康作用于卵巢癌细胞株浆液性卵巢癌细胞系,探讨Survivin蛋白的表达差异及浆液性卵巢癌细胞系侵袭能力的改变。方法用不同浓度的拓扑替康作用于浆液性卵巢癌细胞系细胞,应用免疫组化的方法检测细胞中Survivin蛋白的表达,同时应用体外侵袭系统(Transwell小室),并通过明胶酶谱检测基质金属蛋白酶-2、基质金属蛋白酶-9的表达来检测浆液性卵巢癌细胞系细胞的体外侵袭能力。结果随着拓扑替康浓度的增加,浆液性卵巢癌细胞系细胞的侵袭率降低,各组浆液性卵巢癌细胞系细胞的侵袭率比较具有显著性差异(F=385.885,P<0.05),并且各组基质金属蛋白酶-2、基质金属蛋白酶-9的表达减少,各组基质金属蛋白酶-2、基质金属蛋白酶-9表达的比较具有显著性差异(F分别为263.302、184.378,均P<0.05)。结论拓扑替康可抑制卵巢癌细胞株中Survivin蛋白的表达,从而使细胞侵袭能力下降,这为拓扑替康应用于卵巢癌的治疗提供了一定的理论依据。
Objective To investigate difference in expression of survivin in ovarian cancer cell line and changes in invasion and metastases abilities of cancer cell line SKOV3 when different concentrations of topotecon （TPT） act on the cell line. Methods Different concentrations of TPT acted on SKOV3 cell line in logarithmic growth phase. Immunohistochemistry was used to detect expression of protein survivin, at the same time, semi-quantitative gelatine zymography and Transwell chamber were used to determine changes in invasion and metastasis abilities of SKOV3 cells in vitro. Results With increase in concentration of TPT, the invasion rate of SKOV3 cell line was decreased and there was significant difference between different TPT concentration groups （F = 385. 885, P 〈 0.05）, also, the expressions of MMP-2 and MMP-9 were decreased and there were significant difference between different TPT concentration groups （ F = 263. 302, 184. 378, respectively, both P 〈 0. 05 ）. Conclusion TPT can depress expression of survivin in SKOV3 cell line, then enables invasion and metastases abilitv of the cells decreased. This conclusion provides a theoretical basis for aDvlication of TPT in treatment of ovarian cancer.
出处
《中国妇幼健康研究》
2008年第6期546-549,643,共5页
Chinese Journal of Woman and Child Health Research
