硝基苯降解菌筛选和鉴定

Study on screening and identificatioon of itrobenzene degrading strains

微生物法处理硝基苯废水是较理想的方法。从吉林市某化工厂的排污口的江底底泥采样,以硝基苯为底物,在好氧条件下,经过长时间的驯化、筛选,成功地分离出了4株对硝基苯有较强耐受力的高效降解菌,经鉴定为假单胞杆菌属(Planococcus Migula)、动性球菌属(Pseudomonas Migula)。研究结果表明,在好氧条件下它们能把硝基苯作为惟一的碳源并对硝基苯有较强的生物降解能力;通过驯化可以使降解菌对硝基苯的降解能力有较大地提高。

Microbial treatment is the preferred method to deal with wastewater containing nitrobenzene. Samples were obtained from sediment of the outfall of a chemical factory. Sediment from the riverbed was used for the bacteria source. Under the aerobic conditions and with nitrobenzene as the substrate, four pairs of prior strains which have a strong tolerance on nitrobenzene were separated successfully after a long period of acclimation and screening. They are able to use nitrobenzene as the sole source of carbon and have a good capacity of biodegradation on nitrobenzene and are identified as： Planococcus Migula and Pseudornonas Migula. The results show that under aerobic conditions they can use nitrobenzene as the sole carbon source and have better biodegradability and through bioacclimarion, the biodegradability of bacteria is greatly increased.