PDTC对重度颅脑损伤后NF-κB表达的影响

Expressive Effects of PDTC on the NF-κB after Severe Brain Injury

目的:观察核转录因子-κB(NF-κB)在大鼠创伤性脑损伤(TBI)后脑组织损伤区的表达变化,同时探讨吡咯烷二硫代氨基甲酸酯(PDTC)对其表达的影响。方法:参照改良Feeney自由落体脑损伤装置制备大鼠重度脑损伤模型,将72只大鼠随机分为TBI组、PDTC干预组和假手术对照组,每组分别于伤后2 h、12 h、24 h、48 h断头,取出损伤区脑组织,检测各组的NF-κB mRNA表达水平。结果:TBI组NF-κB于伤后2 h表达增强,12 h表达明显增加,24 h达到高峰,48 h略有下降,与假手术对照组相比差异显著(P<0.05)。PDTC干预组与TBI组相比,NF-κB mRNA表达明显下降,差异显著(P<0.05)。结论:大鼠重度TBI后,NF-κB的活化及过度表达参与了继发性脑损伤过程,PDTC可以通过抑制NF-κB的表达,对TBI起到保护作用。

Objective ：To observe the expressive changes of nuclear factor-kappa B （NF-κB）in cerebral damaged tissue after traumatic brain injury （TBI） in rats and investigate the expressive effects of pyrrolidine dithiocarbamate （PDTC）. Methods： The model of severe brain injury was made by the modified Feeney free-falling-body impact device. Seventy-two rats were randomly divided into TBI group, PDTC prevention group and sham operation group. The rats in each group were killed by decapitation at the point of 2 h, 12 h,24 h and 48 h after TBI respectively to take the brain tissue from the damaged area and detect the expression of NF-κB mRNA in each group by RT-PCR molecular biological technique. Results： The expression of NF-κB heightened at 2 h,while it increased significantly at 12 h and reached the summit at 24 h, but declined at 48 h after injury in TBI group. Compared with the sham operation group, there were significant differences between them （P 〈 0.05 ）. Compared between the PDTC prevention group and the TBI group, the expression of NF-κB mRNA declined significantly and there were significant differences （P 〈 0.05）. Conclusion： After severe traumatic brain injury of rats, the activation and the over expression of NF-κB participate in the processes of the secondary brain injury. PDTC could play a brain tissue protective role of TBI by inhibiting the expression of NF-κB.