大鼠原位肝移植模型制作的若干技术问题

Some Technological Problems on Establishing Orthotopic Liver Transplantation Model in Rats

目的:通过建立稳定的大鼠原位肝移植模型,总结实用性的手术技巧及常见并发症的防治措施。方法:采用改良的二袖套法施行大鼠原位肝移植术123次,观察并分析手术时间、存活率及手术并发症。结果:手术成功率93.5%(115/123),一周存活率为83.7%(103/123),手术并发症主要有麻醉意外、失血性休克、吻合口出血、胆道梗阻、胆漏等。结论:改进的手术方法可以提高手术的成功率,减少并发症的发生。

Objective： To summarize some practical operative techniques and prevention measures for common complications by establishing stable rat orthotopic liver transplantation model. Methods： Orthotopic liver transplantation was performed by modified two-cuff method for 123 times to observe and analyze the operative time, survival rate and operative complications. Results： The successful rate of the operation was 93.5% （115/123 ） and the survival rate of one week was 83.7% （ 103/123 ）. The main operative complications were anesthesia accidents, hemorrhagic shock, stoma bleeding, biliary obstruction, biliary leakage and so on. Conclusion： The modified operative method can heighten the operative successful rate and reduce the occurrence of complications.