流动注射化学发光法测定血浆和尿液中的酒石酸美托洛尔

Determination of metoprolol tartrate in human plasma and urine by flow injection chemiluminescence

酒石酸美托洛尔与Eu^3＋形成的配合物对KMnO4-Na2SO3化学发光反应具有强的增敏作用,据此建立了测定酒石酸美托洛尔的流动注射化学发光新方法.该方法测定酒石酸美托洛尔的线性范围为5.0×10^-7 - 1.0×10^-5g/mL,检出限为1×10^-7g/mL.对浓度为1.0×10^-6g/mL酒石酸美托洛尔溶液连续11次平行测定的相对标准偏差为1.4%.该方法已用于血浆和尿液中酒石酸美托洛尔含量的测定.

In this work, it was found that the weak signal from KMnO4-Na2SO3 reaction was greatly enhanced by Eu^3＋ -metoprolol tartrate complex. Basted on this fact, a new flow injection chemiluminescence method for the determination of metoprolol tartrate was proposed. The linear range and detection limit for determination of metoprololtartrate are 5.0 × 10^-7 - 1.0 × 10^-5 and 1 ×10^-7g/mL, respectively. The relative standard deviation is 1.4%（n=11） for a level of 1.0 × 10^-6g/mL metoprolol tartrate. This method was applied to the determination of metoprolol tartrate in human plasma and urine samples.