肺癌p53蛋白表达及其与淋巴结转移和分期的关系

Expression of p53 protein and its correlation with lymph node metastasis and stages in lung carcinomas

应用链菌素亲生物素-过氧化物酶免疫组化方法观察了p53蛋白在92例肺癌组织的表达情况。结果p53蛋白在肺癌组织中表达的总阳性率为66．3％，正常细胞中未见表达。肺癌有淋巴结转移组p53蛋白的阳性率（75．5％）高于无淋巴结转移组（55．8％）（P＜0．05），Ⅲ～Ⅳ期肺癌p53蛋白的阳性率（82．9％），高于Ⅰ～Ⅱ期肺癌（56．1％）（P＜0．01）。结果表明，p53蛋白是肺癌较特异的肿瘤标记物，其表达与肺癌淋巴结转移及分期有关。

Immunohistochemical analysis of p53 protein was carried out on 92 lung carcinomas in paraffin-embedded sections, using anti-p53 monoclonal antibody DO-1. Nuclear staining was seen in 66. 3 % of lung carcinomas of all histological types. No p53 expression was observed in normal epithelial, stromal or inflammatory cells. 55. 8% of lung carcinomas without lymph node metastasis showed p53 protein expression, while 75. 5% of lung carcinomas with lymph node metastasis showed p53 protein expression, the difference being significant (P＜0. 05).56. 1 % of lung carcinomas at early stage (Ⅰ-Ⅱ) and 82. 9% of lung carcinomas at late stage (Ⅲ - Ⅳ) showed p53 protein expression (P＜0. 01). These findings indicate that p53 protein may be a good marker of lung carcinomas and its expression correlates with lymph node metastasis and tumor progression.