虫害马尾松(Pinus massoniana Lamb)邻枝针叶挥发物及其内源茉莉酸甲酯的快速变化

Rapid changes in volatile compounds and endogenous methyl jasmonates from the adjacent healthy branch needles of damaged Pinus massioniana

对盆栽马尾松针叶进行接虫咬食危害处理后,通过TCT-GC/MS分析了同一株受害枝相邻的健康枝针叶挥发物的成分及相对含量的时序变化。结果表明:萜烯类化合物是邻枝针叶挥发物的主要成分,其次是含氧化合物、含氮化合物等。与对照相比,多数挥发物的相对含量1h略高于对照,2h维持较高水平。同时,用GC/MS分析了邻枝针叶不同时间序列中茉莉酸甲酯的含量,结果表明:虫害马尾松邻枝针叶1h茉莉酸甲酯含量就有所升高,2h显著高于对照,是对照的近1倍。证明马尾松受到虫害后,启动了体内的防御系统,并诱导邻枝产生抗性。

After one branch needles of potted plant Pinus massioniana was fed by Dendrolimus Punctatus, the composition and relative contents of volatile organic compounds （ VOCs ） from the adjacent upper branch needles of insect-damaged branch at different times was analyzed by TCT-GC/MS. The results showed that terpenes were the dominant, while the others such as oxygenated and nitrogenous compounds were less abundant VOCs. Most of VOCs in treated plants had higher contents than those in the control after 1 h treatment and remained much high levels at 2 h. The content of methyl jasmonic acids （MeJA） in adjacent needles of infected Pinus massioniana was increased after 1 h treatment and nearly doubled that of control plants at 2h. The data indicates that the defensive system of Pinus massioniana was initiated and leading to the resistance reaction of the neighborhood undamaged branches by inset-pest.