鼻咽癌高温固化瘤苗免疫动物在高压氧暴露下的实验研究

The Animal Experimentation Study on Animal Nasopharyngeai Neoplasms of Megatemperature Ultrasound Solidification Tumor Vaccine on Hyperbaric Oxygen Condition

目的探讨在高压氧环境下鼻咽癌高温固化瘤苗免疫小鼠的抗肿瘤效应及对免疫功能的影响。方法将实验小鼠随机分为对照组、常压瘤苗组和高压氧瘤苗组，采用鼻咽癌高温固化瘤苗对小鼠进行免疫实验，通过癌细胞攻击实验和胸腺细胞自发性^3H—TdR掺入量测定，观察其在小鼠体内诱发的抗瘤和胸腺免疫效应。结果A组的抑瘤率为O，B组和C组的抑瘤率分别为80．00％和93．33％，死亡率分别为86．67％、20．00％和6．67％，生命延长率分别为00．00％、75％和87％，6周生存天数（19．84±1．5）、（38．91±4．3）d和（52．31±4．1）d，肿瘤发生率分别为100．00％、40．00％和26．67％，其两组与A组比较有显著差异（P〈0．05），而B组和c组比较有明显差异（P〈0．05）。免疫B组和C组的腺细胞平均数显著上升，而胸腺细胞’H—TdR掺入量明显下降。结论免疫小鼠可获得对随后接种同源细胞攻击，有免疫保护作用，并产生免疫记忆；高压氧曝露的免疫小鼠的抗肿瘤效应和免疫力明显强于另外两组，高温固化瘤苗与高压氧结合能显著地提高激活宿主免疫系统，抑制肿瘤生长和延长生命，有望成为一种新的、安全的、有效和、无毒副作用的综合治疗新模式。

Objective To investigate the anti - cancer results and immunizing effect of ultrasound solidification tumor vaccine of mouse in hyperbaric oxygen circumstance. Methods The hundreds of mouse randomization into control group, ordinary pressure tumour vaccine group, hyperbaric oxygenation ultrasound solidification tumor vaccine group. The immunizing test was taken on ultrasound solidification tumor vaccine circumstance. Results The total remission rate of tumor in A groups, B groups and C group were 0% ,80. 00% and 93.33% respectively. The mortality rate were 86. 67% ,20.00%and 6. 67% in B groups and C group respectively. The life span prolongation rate were100. 00% 、 75% and 87 % respectively. The mean survival time were （ 19. 84 ± 1.5 ） days, （ 38.91 ± 4. 3 ） days and （ 52. 31 ± 4. 1 ） days respectively. The tumorigenesis rate were 100. 00% ,40.00% and 26. 67% respectively. The B, C group vs A group the difference is remarkable in statistics （ P 〈 0. 05 ）. The number of immunizing glandular cell increase remarkable in B group and C group. The ^3 H - TdR incorporate rate of thymus cell decrease obviously. Conclusions The immunized mouse may have the ability of attack the late inoculated isogenic cell. In other words, It can get immunoprotection and produce immunological memory. The immunity and anti - cancer ability of immunized mouse on hyperbaric oxygen condition better than other goups. The combination of hyperbaric oxygen therapy with ultrasound solidification tumor vaccine get better therapeutic effect and lower side effects than the control group. It may activating the host immunologic system. It is to be worth to generalization.