AcA、AsAb、EmAb、BA抗体与反复自然流产关系的研究

Research on relationship between AcA,AsAb,EmAb,BA and recurrent spontaneous abortion

目的探讨抗心磷脂抗体(AcA)、抗精子抗体(AsAb)、抗子宫内膜抗体(EmAb)、封闭抗体(BA)与反复自然流产(RSA)的关系。方法采用酶联免疫吸附法(ELISA法)测定112例反复自然流产患者(流产组)血清中BA抗体,AcA、AsAb、EmAb项目的测定采用金标免疫斑点法测定。结果流产组AcA、AsAb、EmAb阳性率分别为37.50%、25.00%、31.25%,明显高于对照组(分别为7.14%、4.08%、6.12%),差异都具有统计学意义(P<0.01);而流产组BA抗体的阴性率为89.29%,明显高于对照组22.45%,两者差异具有统计学意义(P<0.01)。结论AcA、AsAb、Em-Ab、BA抗体与反复自然流产有着密切的关系,且BA抗体的缺乏是引起RSA患者最常见的免疫因素之一。

Objective To study the relationship between anticardiolipin antibody （AeA）, antisperm antibody （AsAb）, anti-endometrium antibody （EmAb）, blocking antibody （BA） and recurrent spontaneous abortion. Methods Serum BA was measured by ELISA; serum AcA, AsAb and EmAb were measured by gold-labeled immunodotting assay. Results The positive rate of AcA, AsAb and EmAb in abortion group were significantly higher than those of control group （37. 50% vs 7.14%, 25.00% vs 4. 08%, 31. 25% vs 6. 12%, respectively）; and the negative rate of BA was 89.29% in abortion group, significantly higher than that of control group （22.45%） （P〈0. 005）. Conclusion The results show that AcA, AsAb, EmAb, BA in serum may be closely related to recurrent spontaneous abortion and a lack of BA is one of the most common immune factors in RSA.