达斡尔族谷胱甘肽S-转移酶GSTT1和GSTM1基因多态性

Frequencies of glutathione S-transferase gene (GSTT1 and GSTM1) polymorphism in a Daur population

目的探讨内蒙古地区达斡尔族谷胱甘肽S-转移酶(glutathione S-transferases,GSTs)GSTM1和GSTT1基因多态性分布特点,为内蒙古少数民族基因型研究提供相关数据。方法采用内对照聚合酶链反应技术(PCR)和凝胶成像分析方法,对220例内蒙古地区达斡尔族个体的GSTT1、GSTM1基因缺失型频率进行了分析。结果GSTM1基因缺失型、GSTT1缺失型在内蒙古地区达斡尔族人群中检出频率分别为50.8%和71.4%。同时具有GSTM1缺失型和TSTT1缺失型个体的检出频率为31.4%。结论中国达斡尔族人群GSTM1、GSTT1基因呈多态性分布,与汉族相比存在一定差异,与蒙古族相比差异无统计学意义。

Objective To explore the distrbution characteristics of glutathione S transferase T1 and M1 （GSTT1 and GSTM1） gene polymorphism of Daur population in Inner Mongolia region, and provide the relevant data for studying the genotype of minority ethnic group of the Inner Mongolia. Methods Genomic DNA was extracted from peripheral blood and the fragments of GSTM1 and GSTT1 was amplified by internal control allele specific PCR in 220 volunteers who had been residents in Inner Mongolia region. At the same time, the genotype of GSTT1 and GSTM1 was resolved by the density of 2. 5% agarose gel electrophoresis. Results In the studied population, the frequencies of GSTM1 （ - ） and GSTT1 （-） were 50.8 % and 71.4 % respectively. The frequency of the individuals with combination of GSTM1 （- ） and GSTT1 （-） was 31. 4%. Conclusion The distribution of GSTM1 and GSTT1 displays gene polymorphism in Chinese Dour population, approximate to Mongo lion population in the north of China, but there is significant difference between Chinese Daur population and Han people.