兔骨髓基质细胞和骨血肿块移植促进骨折愈合的研究

Study on combined transplantation of rabbit's marrow stroma cells and hematomas in bone healing

目的:研究骨折后骨血肿块和骨髓基质细胞的骨再生潜能,以及骨血肿块和骨髓基质细胞联合移植对骨折愈合的影响,探讨促进骨折愈合的新途径。方法:在新西兰兔胫骨上制造骨缺损模型,3d后取出骨血肿,并从股骨抽骨髓血分离出骨髓基质细胞,将骨髓基质细胞联合骨血肿块回植到实验组骨缺损区;对照组骨缺损区则单纯行骨血肿块回植。定期拍X线片和取出骨痂行组织学观察。结果:骨缺损处骨痂生成数量在实验组和对照组两组间有显著性差异,骨髓基质细胞联合骨血肿块移植组的骨痂形成数量和骨膜反应明显多于以单纯骨血肿块移植组;组织学观察:实验组成骨样细胞形成数量也明显多于对照组。结论:骨髓基质细胞有较强的骨再生潜能,骨髓基质细胞和骨血肿块联合移植比单纯骨血肿移植成骨效果更好。

Objective ： To study the potentiality of osteanagenesis of the hematomas formed around the fractures and that of the marrow stroma cells, evaluate the effect of the combined trans-plantation of the hematoma and the marrow stroma cells, to explore a new method to accelerate the union of fracture. Methods： The bone defect models were made on the tibias of the New-Zealand＇s rabbits. The hematomas formed around the fracture were taken out 3 days latter after the operation, the marrow stroma cells were abstracted from the femoral marrow simultaneously. And then the mixture of the hematoma and the marrow stroma cells were transplanted to the defects of the tibias in the experiment group, and the hematoma transplanted simply to the same place in the control group. The radio-graph and the histological observation of the osteotylus were carried out regularly post-operation. Results： ①There was a significant difference in osteotylus quantity between the two groups ： more osteotylus and obvious periosteal proliferation were found in the experiment group than that in the control group which accepted the transplantation of the hematomas alone. ②There was a significant difference in osteoblast number between the two groups ： more sclerotomal-like cells were observed under the microscope in the experiment group than that in the control group. Conclusion： Marrow stroma cells have great potentiality of osteoanagenesis. The result of combined transplantation of the marrow stroma cells and the hematomas is more effective than that of simple transplantation of the bone hematoma.