复方积雪草合剂对IgA肾病大鼠肾组织炎症因子和ECM表达的影响

The Effects of Centella Asiatica Compound on Renal Expression of Cytokines and ECM in Rats with IgAN

目的:探讨复方积雪草合剂对实验性IgA肾病(IgAN)模型大鼠肾脏组织细胞外基质(ECM)和炎症因子表达的干预作用。方法:采用改良口服牛血清白蛋白(BSA)和尾静脉注射葡萄球菌肠毒素B(SEB)的免疫复合法建立大鼠实验性IgAN模型,40只大鼠随机分为正常组、模型组及复方积雪草合剂治疗组(设高、中、低3个剂量)。药物干预12周,分别观察实验大鼠尿蛋白、肾功能、血尿及肾病理的变化,并用免疫组化及半定量法检测肾组织中FN、IL-6、TGF-β1的含量。结果:复方积雪草治疗组能显著减少实验大鼠尿红细胞和尿蛋白(P<0.05),同时对实验大鼠肾组织TGF-β1、FN的表达均有明显抑制作用(P<0.05),但对IL-6的表达未见显著作用,且无明显剂量依从性。结论:复方积雪草合剂对IgAN模型鼠具有显著的治疗作用,能有效减少蛋白尿和红细胞,减轻肾组织的免疫损伤。其作用机制可能与其抑制或降低炎症因子的分泌有关,并最终延缓IgA肾病的发展。

Objective： To investgate the effects of centella asiatica compound on renal expression of cytokines and ECM in rats with IgAN. Methods：Rats model of IgAN were induced with an improved immune method,oral adminstration of BSA and injection of SEB via tail vein. Fourty rats were randomly divided into normal, IgAN model and centella asiatica treatment groups, and then divided further into three sub - groups： high dose, medium dose and low dose. At the 12th week, kidney pathology, serum creatinine （Scr）, blood urea nitrogen （BUN）, urinary erythrocyte and protein excretion were observed. The content of TGF - β1 and FN in renal cortex tissues were determined by immunohistochemistry, and IL-6 in renal tissue were detected by ELISA. Results： Red blood cell and protein in urine were significantly decreased after treatment of centella asiatica compound（P〈 0.05）, and the expression of TGF-β1 and FN in renal cortex tissue were also siginificantly decreased（P 〈 0.05）. But there were no significant different in the expression of IL- 6 between model and treatment groups. Conclusion： This study provides the first direct evidence that Centella asiatica compound can repair renal injury and decrease excetion of urine protein and red blood cell in rats with IgAN, the mechanism of which may base on that Centella asiatica compound can inhabit and or decrease secretion of cytokines in renal cortex, and finally delay the progress of IgAN.