山莨菪碱与旋覆花素及苦碟子对过度训练大鼠肾组织上皮钙黏素表达的影响

The Effects of Anisodamine,Inula Britannica and Ixeris Sonchifolia Hance on the Expression of Renal Tissue E-Cadherin in Exhausted Rats

目的:探讨过度训练大鼠肾组织上皮钙黏素表达的变化及山莨菪碱、旋覆花素、苦碟子干预的影响。方法:将80只雄性Wistar大鼠随机分为安静对照组(CN)、力竭运动组(ES)、山莨菪碱干预组(AD)、旋覆花素干预组(IB)、苦碟子干预组(IS)。CN组为安静对照;ES组又根据力竭后恢复时间分为力竭即刻(ESI)、力竭后6h(ES6h)和力竭后24h(ES24h);AD组、IB组、IS组均分别于力竭后6h和力竭后24h取材观察各项指标。每个时间点8只大鼠。采用大鼠游泳至力竭建立过度训练模型。采用免疫组织化学法检测各组肾组织E-Cadherin蛋白表达的变化;采用CMIAS病理图像分析仪测量E-Cadherin蛋白表达的平均光密度。结果:对照组大鼠肾组织有E-Cadherin的丰富表达,广泛分布于肾小管上皮细胞膜上及细胞浆内;力竭即刻、6h及24hE-Cadherin表达逐渐减弱(P<0.05)。山莨菪碱、旋覆花素和苦碟子组大鼠肾组织E-Cadherin表达比同期力竭组明显增强(P<0.05)。结论:过度训练可引起肾组织E-Cadherin的表达下调,山莨菪碱、旋覆花素和苦碟子干预后可逆转这种变化,这可能是过度训练引起急性肾损伤及上述3种药物减轻OTIAKI的重要机制之一。

Objective： To observe the changes of the expression of renal tissue E- Cadherin and the influences of Anisodamine, Inula Britannica and Ixeris Sonchifolia Hance in overtraininginduced acute kidney injury（OTIAKI） rats, and to investigate the role of E- Cadherin signal pathway in the development of OTIAKI. Methods：Eighty male Wistar rats were randomly divided into five groups： control group（CN, n = 8）, exhaustive swimming（ES, n = 24）, Anisodamine（AD, n = 16）, Inula Britannica（ IB, n = 16） and Ixeris Sonchifolia（IS, n = 16）. The rats of CN were quiet and no swimming. The rats of ES swimed to exhaustive state and sacrificed at immediately （ESI） ,6 hour （ES 6 h） and 24 hour （ES 24 h） after exhaustive swimming. The rats of AD received intraperitoneal injection of Anisodamine at the dose of 10 mg/kg body weight at 20 minutes before swimming and thenswimed to exhaustive condition. The rats of IB took orally Inula Britannica at the dc,se of 25 ml/kg body weight at 24 h before swimming and then swimed to exhaustive condition. The rats of IS received intraperitoneal injection of Ixeris Sonchifolia Hance at the dose of 20 g/kg body weight at 20 minutes before swimming and then swimed to exhaustive condition. The rats of AD, IB and IS group were sacririced at 6 hour （AD 6 h, IB 6 h, IS 6 h） and 24 hour （AD 24 h, IB 24 h, IS 24 h） after exhaustive swimming. The animal model of overtraining- induced acute kidney injury was developed by exhaustive swimming. The expression of E - Cadherin in renal tissue were observed by immunohistochemistry. Results： Immunohistochemistry staining showed that the obvious positive expressions of E - Cadherin were observed in renal tubular epithelial cells of cotex and medullar area in CN rats. The expressions of E - Cadherin decreased progressively in ESI, ES 6 h, ES 24 h rats （P 〈 0.05）. The expressions of E - Cadherin increased remarkably in AD, IB and IS rats compared with ES rats（P 〈 0.05）. Conclusion： Overtraining could induce the down- regulation of expressions of renal tissue E - Cadherin. Pretreatment with Anisodamine, Inula Britannica and Ixeris Sonchifolia Hance could reverse the changes of expressions of renal tissue E Cadherin induced by exhaustive swimming.