脑缺血后cNOS mRNA表达:NO在脑缺血早期的保护作用

Changes of cNOS mRNA Gene Expression After Cerebral Ischemia: the Protective Effect of NO on Cerebral Ischemia in the Early Stage

在SHR MCAO局灶脑缺血模型基础上,分别测定脑缺血后不同时限NOS活性变化及cNOS mRNA表达水平,并应用外源性NOS抑制剂L-NNA,观察NO代谢变化对脑缺血脑水肿的影响。结果发现脑缺血早期cNOS mRNA表达增加,NOS活性增高,L-NNA则促进脑水肿发生,提示NO在脑缺血早期可能具有保护作用。

On the basis of model of MCAO in spontaneously hypertensive rats (SHR), the changesof NOS activity and the NOS mRNA gene expression level were determined at different ischemictime points, and the effects of NO metabolism on ischemic brain edema by using extrinsicNOS inhibitor (L-NNA) were also explored. The results showed that the expression of NOS mRNA increased in the early ischemic phase, the activity of NOS after cerebral ischemia were significantly increased and the L-NNA can promote the brain edema. These resultssuggested that NO might have some protective effect on ischemic brain tissue in the early ischemicstage.