摘要
目的:探讨褪黑素(MT)对大鼠急性肺损伤(ALI)时肺脏的保护作用及其可能机制。方法:将大鼠随机分为4组:对照组;脂多糖(LPS)组;地塞米松(DEX)和MT处理组。各组动物分别于气道内滴注后3、6和12h检测肺组织髓过氧化物酶(MPO)和超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;免疫组化检测细胞间黏附分子-1(ICAM-1)在肺组织的表达。结果:LPS组SOD活性较对照组明显降低(P<0.01),而MPO活性与MDA含量以及ICAM-1的表达则显著升高(P<0.01);应用MT及DEX均显著缓解上述变化(P<0.05或P<0.01)。结论:MT对ALI时的肺脏起明显的保护作用,其机制可能与MT清除自由基及抑制ICAM-1的表达有关。
Aim: To investigate the protective effect of melatonin(MT) on lung tissues during acute lung injury(ALI) in rats and its possible mechanism Methods: All rats were randomly divided into four groups: control group, lipopolysaccharide (LPS) group, dexamethasone(DEX) and MT treatment group. Myeloperoxidase (MPO) activity, superoxide dismutase (SOD) activity and malonaldehyde(MDA) content of lung tissues were detected at 3,6 and 12 h after intratracheal instillation in each group. In addition, the expression of intercellular adhesion molecule-1 ( ICAM-1 ) were observed through immunohistochemistry staining in lung tissues. Results: Compared with control group, SOD activity decreased significantly in LPS group(P〈 0.01), but MPO activity, MDA content and the expression of ICAM-1 increased obviously(P 〈 0.01 ). The administration of MT and DEX mitigated above changes significantly(P 〈 0.05 or P 〈 0.01). Conclusion: MT possessed protective effect on lung tissues during ALI through scavenging free radicals and inhibiting the expression of ICAM-1 probably.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2008年第4期464-467,I0011,共5页
Chinese Journal of Applied Physiology
基金
河北省科技厅资助项目(04276101D-45)