摘要
利用HPLC方法,对中国淫羊藿属Epimedium,主要是Epimedium亚属Diphyllon组的35种1变种129个植物叶片样品进行了淫羊藿苷类黄酮化合物的HPLC指纹图谱特征分析。主要依据样品图谱中的第二组峰'ABCI'组峰特征将所有图谱归为4大类9亚类,通过与花形态性状的关联分析,确定不同类型图谱以及包含色谱峰的演化关系,并且提出:II-3是最原始的类型,II-1、IV、I-3为原始类型直接衍生的类型;I-1为基本类型,I-2、I-4、III、II-2为进化或者是特化类型。HPLC图谱类型的划分与W.T.Stearn2002年发表的淫羊藿属分类系统Diphyllon组下4个系的划分具有较好的相关性。
In this paper, 129 leaf samples from 35 species and one variety of the Chinese Epimedium (Berberidaceae), most of which were placed under subgen. Epimedium and sect. Diphyllon, were analyzed by HPLC method. The HPLC chromatogram profiles of all the samples for icariin and similar compounds were achieved, sorted and analyzed. According to the second peak group (named as "ABCI" peak group) characters, chromatograms were divided into four main types and nine subtypes. By correlation analysis with flower morphology, II-3 was suggested to be the most primitive type; II-I, IV and I-3 were primitive and closely related to II-3; I-I was basic type; I-2, I-4, III and II-2 were derived types. The HPLC chromatogram type division corresponds to W. T. Stearn's classification on sect. Diphyllon with four series in 2002.
出处
《植物分类学报》
CSCD
北大核心
2008年第6期874-885,共12页
Acta Phytotaxonomica Sinica
基金
国家自然科学基金项目(30270101
30530860)资助。