Eppin抗原的二级结构分析B细胞表位预测

Prediction of B cell epitope for Eppin antigen

目的分析Eppin抗原的二级结构并预测其B细胞表位。方法联合运用多种方法对Eppin的二级结构和表面特性,如亲水性、理化性质、可及性、免疫原性和可塑性等方面进行分析,预测其抗原表位。结果Eppin存在多个潜在的抗原表位位点,可能的蛋白质抗原表位区域:14～23,30～44,48～54,56～68,78～85,97～106,109～116,125～132。体外实验证明,所预测抗原表位区域基本能与免疫抗血清发生抗原特异性反应。结论应用多参数成功预测了Eppin抗原的B细胞表位。

Objective To predict the B cell epitope for Eppin antigen. Methods The secondary structure and surface properties of Eppin, such as physical and chemical properties, hydrophilicity, accessibility, antigenicity and plasticity, were analyzed by a variety of methods. Results Many distinct antigenic epitopes in Eppin were identified by computation, the possible location of which is in the regions of 14 - 23,30 - 44, 48 - 54, 56-68, 78 -85, 97 -106, 109 -116, and 125 -132. Conclusion The B cell epitope for Eppin is successfully predicted.