黄芪、莪术配伍对胃癌细胞COX-2表达的调节作用

Regulatory effect of compatibility of Astragalus membranaceus and Rhizoma curcumae on COX-2 expression in gastric cancer MKN-45 cells

目的:观察黄芪、莪术配伍对MKN-45细胞COX-2、PPARγ、NF-κB的影响.方法:以黄芪、莪术配伍作用于MKN-45细胞,并设塞莱希布组、罗格列酮组、黄芪组、莪术组和空白组相对照.用MTT法观察各组药物对胃癌细胞的抑制率,用RT-PCR以及Western blot方法检测给药后人胃癌细胞COX-2、PPARγ、NF-κB基因及COX-2蛋白表达的变化.结果:各组对COX-2和NF-κB的mRNA表达均有抑制作用,除莪术外对PPARγ mRNA均有促表达作用,其中以塞莱希布组和配伍组对COX-2 mRNA表达的抑制作用最为明显,且配伍组作用明显强于黄芪组和莪术组,以罗格列酮组和配伍组对NF-κB mRNA的表达抑制作用和对PPARγ表达的促表达作用都是最明显.结论:黄芪、莪术配伍能起到增效作用,对细胞的抑制效应明显,对COX-2的抑制作用大于黄芪、莪术的单独效应,与塞莱希布相近,其对COX-2的抑制作用可能是通过PPARγ/NF-κB信号途径发挥作用的.

AIM： To investigate the effect of Astragalus membranaceus and Rhizoma curcumae＇s compatibility on MKN-45 cells and the regulatory action of the compatibility on expression of cyclooxygenase 2 （COX-2）, peroxisome proliferators activated receptorγ （PPARγ） and nuclear factor κB （NF-κB）. METHODS： The compatibility of Astragalus membranaceus and Rhizoma curcumae was used on MKN-45 cells and there were five groups including Celecoxib group, Rosiglitazone group, Astragalus membranaceus group, Rhizoma curcumae group and control group. The inhibition ratio in each group was determined using MTT method, and the expressions of COX-2 mRNA, PPARγ mRNA, NF-κB mRNA and COX-2 protein were measured using RTPCR and Western blot methods. RESULTS： Both the Chinese drugs and Western medicines had suppression on NF-κB mRNA and COX-2 mRNA. All medicines except Rhizoma curcumae promoted the expression of PPARγ, mRNA. The most obvious suppressive effect on COX-2 mRNA expression was detected in celecoxib group and compatibility group. And suppressive effect was significantly stronger in compatibility group than either in Astragalus membranaceus group or Rhizoma curcumae group. Both Rosiglitazone group and compatibility group had the best suppressive effect on NF-κB mRNA and the best promoting effect on PPARγ mRNA. CONCLUSION： Astragalus membranaceus and Rhizoma curcumae＇s compatibility has better effect with marked suppressive effect. The compatibility group showed stronger suppressive effect on COX-2 expression than Astragalus membranaceus and Rhizoma curcumae used alone, closing to Celecoxib. Its suppressive effect on COX-2 may be produced through the signal pathway of PPART/NF-κB.