摘要
目的:探讨人血管生成素-1(angiopoietin-1,Ang1)对体外培养的人胃癌细胞MGC-803所产生的凋亡抑制作用的可能机制.方法:以适当感染复数(MOI=20)的重组腺病毒Ad-Ang1和对照病毒Ad-GFP感染人胃癌细胞,对照组用无血清培养基培养,并分别通过RT-PCR、Western blot方法检测Bcl-2及Bax mRNA和蛋白的表达.结果:Bcl-2 mRNA和蛋白的表达量在Ad-Ang1组中明显高于Ad-GFP组及对照组(0.609±0.01vs0.462±0.02,0.609±0.01vs0.475±0.02,均P<0.05),Ad-GFP组与对照组相比无显著性差异;而Ad-Ang1组的BaxmRNA和蛋白的表达量则低于Ad-GFP组及对照组(0.313±0.04vs0.413±0.02,0.313±0.04vs0.407±0.03,均P<0.05),后两组相比无显著性差异;Bcl-2/Bax比值在Ad-Ang1组中也明显增高.结论:Ang1基因转染体外培养的人胃癌细胞后,不论是在转录水平还是在翻译水平均上调了细胞Bcl-2的表达,同时使Bax表达下调,Bcl-2/Bax比值增大,此途径可能为Ang1抑制血浆饥饿所诱导的肿瘤细胞凋亡的机制之一.
AIM： To explore the possible mechanism underlying the inhibitory effect of angiopoietin-1 （Ang1） on apoptosis of human gastric cancer cells MGC-803.
METHODS： The human gastric cancer cells （MGC-803） were cultured with Ad-Ang1 and Ad- GFP at proper multiplicity of infection （MOI = 20） and the expression levels of bcl-2 mRNA, bax mRNA and Bcl-2 protein, Bax protein were determined using RT-PCR and Western blot, respectively.
RESULTS： The expression levels of Bcl-2 mRNA and its protein were higher in Ad-Ang1 trans- fected group than in Ad-GFP transfected group and control group （0.609 ± 0.01 vs 0.462 ± 0.02, 0.609 ± 0.01 vs 0.475 ± 0.02, both P 〈 0.05）. There was no significant difference between Ad-GFP group and control group. However, compared with the other groups, the expression level of Bax mRNA and its protein in the MGC-803 cells treated with Ad-Ang1 were significantly downregulated （0.313 ± 0.04 vs 0.413 ± 0.02, 0.313 ± 0.04 vs 0.407 ± 0.03, both P 〈 0.05）. Meanwhile, the ratio of Bcl-2 to Bax increased markedly in Ad- Ang-1 transfected group.
CONCLUSION： Ang1 gene can significantly upregulate Bcl-2 expression and down-regulate Bax expression at both transcriptional and translational levels in vitro, which may be one of mechanisms underlying protection against serum starvation-induced apoptosis.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第32期3605-3609,共5页
World Chinese Journal of Digestology
