TGFβ1和CTGF在非酒精性脂肪性肝炎肝组织中的表达及意义

Expression and its significance of connective tissue growth factor and transforming growth factorβ1 in hepatic tissues of non-alcoholic steatohepatitis

目的:探讨非酒精性脂肪性肝炎(NASH)患者肝脏转化生长因子β1(TGFβ1)和结缔组织生长因子(CTGF)的表达及二者在脂肪性纤维化发展过程中的相关性.方法:采用HE染色,光镜下观察21例非酒精性脂肪性肝炎组(NASH组)肝组织和10例正常肝组织组标本肝组织的病理改变;RT-PCR及免疫组织化学技术分别检测以上两组标本中TGFβ1和CTGF的表达情况.结果:光镜下非酒精性脂肪性肝炎组出现明显炎症反应和纤维化.免疫组化结果显示非酒精性脂肪性肝炎组肝脏TGFβ1和CTGF的表达显著高于正常肝脏组(109.52±4.50vs114.47±2.00,117.07±3.60vs125.05±3.37,均P<0.01).NASH组TGFβ1和CTGFmRNA的表达水平升高明显,与对照组比较差异有统计学意义(0.66±0.07vs0.46±0.04,0.59±0.08vs0.41±0.05,均P<0.05).结论:TGFβ1和CTGF在非酒精性脂肪性肝炎中的表达升高可能参与了脂肪性肝炎向肝纤维化转变,CTGF可能在诊断和治疗非酒精性脂肪性肝炎中扮演重要的作用.

AIM： To investigate the expression of connective tissue growth factor （CTGF） and transforming growth factorβ1 （TGFβ1） in non-alcoholic steatohepatitis hepatic tissue and to explore correlation between them during hepatic fibrosis. METHODS： HE staining, RT-PCR and immuno-histochemistry methods were used to measure TGFβ1 and CTGF mRNA expression levels in liver tissue of 21 patients with non-alcoholic steatohepatitis and 10 normal controls meanwhile hepatic histopathological changes were evaluated. RESULTS： The optical microscopic analysis indicated that liver inflammation and fibrosis were apparently present in non-alcoholic steatohepatitis group （NASH group）. And immunohistochemistry analysis revealed that the expressions of TGFβ1 and CTGF in hepatic tissue were significantly higher in NASH group than in normal control group （109.52 ± 4.50 vs 114.47 ± 2.00, 117.07 ± 3.60 vs 125.05 ± 3.37, both P 〈 0.01）. TGFβ1 mRNA and CTGF mRNA expression were enhanced significantly in NASH group, compared with the control group （0.66 ± 0.07 vs 0.46 ± 0.04, 0.59 ± 0.08 vs 0.41 ± 0.05, both P 〈 0.05）. CONCLUSION： The enhanced expressions of TGFβ1 and CTGF in hepatic liver tissue of non-alcoholic steatohepatitis patients might be involved in fatty fibrosis. CTGF plays an important role in diagnosis and treatment of nonalcoholic fatty liver disease.