PLFAs稳定同位素技术及其在土壤微生物学中的应用

Stable Isotope Techniques of PLFAs and It’s Application in Soil Microbiology

磷脂脂肪酸(PLFAs)是微生物细胞膜的重要组分,可作为活体微生物的生物标记物。稳定同位素技术与生物标记物相结合为揭示微生物种群结构及其功能提供了一种有效的方法,可用来阐明复杂土壤生态系统中微生物源有机质代谢途径以及个别微生物种群特征,将特定微生物种群与相应生物化学过程相联系。介绍了PLFAs稳定同位素分析技术,包括气相色谱-燃烧-同位素比例质谱(GC-c-IRMS),气相色谱-质谱联机(GC-MS)以及核磁共振(NMR),并描述上述方法在土壤微生物学中的应用以及其优缺点。

PLFAs are important components of cell membrane and can be used as biomarkers of active microorganisms. The application of stable isotope techniques in PLFAs＇ analysis could be an effective way to elucidate the community structures and functions of soil microbiology. These techniques are used to illuminate the metabolic pathway of microbial organic matter, identify the characteristics of particular microbial communities and link directly microbial populations with the corresponding biochemistry process. In this review, three different stable isotope techniques of PLFAs analysis had been introduced, including GC-c-IRMS （gas chromatography combustion isotope ratio mass spectrometry）, GC-MS （gas chromatography mass spectrometry） and NMR （nuclear magnetic resonance） techniques. These techniques＇ applications in soil microbiology were also introduced.