睾酮对1,25-(OH)_2D_3抑制LoVo细胞增殖的影响及大肠癌患者血清睾酮水平的变化

The Influence of Testosterone on the Inhibitory Effect of 1,25-(OH)_2D_3 on the Proliferation of LoVo Cell and the Changes of Serum Testosterone in Patients with Colorectal Cancer

观察睾酮对1,25-(OH)_2D_3抑制LoVo细胞增殖的影响及大肠癌患者血清睾酮水平的变化。方法:本研究采用细胞培养方法,观察1,25-(OH)_2D_3及睾酮(T)对培养的大肠癌LoVo细胞株增殖的影响,在此基础上,又观察了大肠癌患者血清T水平的变化。结果:1,25-(OH)_2D_3对LoVo细胞有明显的增殖抑制作用,这种作用具有浓度及时间依赖性,当11,25-(OH)_2D_3浓度达10^(-8)mol/L时,其抑制串可达30％。T单独作用时,对LoVo细胞的增殖无明显作用。但T与1,25-(OH)_2D_3合用时,可使后者对细胞增殖的抑制作用丧失。男性大肠癌患者较对照血清T水平明显降低(P<0.05),术后可回升接近正常水平。结论:T可拮抗1,25-(OH)_2D_3对LoVo细胞的增殖抑制效应,血清T水平可作为结直肠癌患者临床观测的指标之一。

Aims:To evaluate and observe the influence of testosterone on the inhibitory effect of l,25-(OH)2D3 in the proliferation of LoVo cell and the changes of serum testosterone level in colorectal cancer patients. Methods: The effects of 1,25-(OH)2D3 on the proliferation of LoVo colon cancer cell line were observe by cell culture method, and the testosterone level was determined in colorectal cancer patient. Results: 1,25-(OH)2D3 had a significant inhibitory effect on the proliferation of LoVo cells dose- and time-dependantly. The inhibitory rate was 30% when the concentration of 1,25-(OH)2D3 reached 10^(-8) mol/L. Testosterone had no obvious effects on the proliferation of LoVo cells. On the contrary, testosterone(10^(-6) mol/L) would cancel the inhibitory effect of 1,25-(OH)2D3(10^(-7) mol/L). In male colorectal cancer patients, there was a low serum testosterone level compared with the control group(P<0.05). The lowered testosterone level would rise to normal level after radical excision of colorectal cancers in the patients. Conclusions: Testosterone antagonised the inhibitory effect of 1,25-(OH)2D3 on the proliferation of LoVo cells and serum testosterone could be a marker of colorectal cancer.