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肿瘤坏死因子对肠上皮细胞分泌片的影响 被引量：3

Influence of TNF-α on the expression of secretory component in Caco-2
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摘要 目的:研究TNF-α对Caco-2细胞表达SC的影响。方法:采用免疫组化、ELISA、Western blot、Real-time PCR方法检测肠上皮细胞表达SC的变化。结果:与无TNF-α刺激比较,用不同浓度TNF-α刺激后SC阳性细胞、培养上清液中游离SC、Caco-2细胞SC蛋白和SC基因的表达明显增加(P<0.01)。结论:TNF-α上调Caco-2细胞内SC的表达。 Objective：To study the influence of TNF-α on the expression of secretory component （SC） in Caco-2 cells. Methods： Immunocytochemistry,ELISA, Western blot and quantitative real-time PCR were used to test SC-positive cells of Caco-2, free SC in culture super- natants, protein and mRNA expression of SC in the cells. Results： The increase of SC-positive cells, free SC in culture supematants, SC protein expression and expression of SC genes in Caco-2 cells was found under stimulation of TNF-α treatment compared with control（ P 〈 0.01 ）. Con. dusion： TNF-α upreglulats the expression of secretory component in Caco-2 cells.
作者 刘冬妍 刘沛
机构地区 中国医科大学附属盛京医院实验研究中心
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2008年第11期993-996,共4页 Chinese Journal of Immunology
基金 国家自然基金资助项目(No.30670947)(No.30871158) 辽宁省教委课题资助项目(No.05L466)(No.2008783)
关键词 分泌片 多聚免疫球蛋白受体 肿瘤坏死因子 CACO-2细胞 Secretory component（SC） Polymeric Ig receptor （pig, R） TNF-α Caco-2
分类号 R446.62 [医药卫生—诊断学]
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参考文献23

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同被引文献19

  • 1朱淑敏,郑一宁,孙燕.PEG-ELS与甘露醇用于结肠镜检查术前准备的对比[J].现代护理,2006,12(8):714-715. 被引量：28
  • 2Bruno M E C,Kaetzel C S.Long-term exposure of the HT-29 human intestinal epithelial cell line to TNF-α causes sustained up-regulation of the polymeric Ig receptor and proinflammatory genes through transcriptional and posttranscriptional mechanisms[J].J Immunol,2005;174:7278-7284.
  • 3Yokomizo A,Moriwaki M.Transepithelial permeability of myricitrin and its degradation by simulated digestion in human intestinal Caco-2 cell monolayer[J].Biosci Biotechnol Biochem,2005;6:1774-1776.
  • 4Takenouchi-ohkubo N,Asano M,Chihaya H et al.Retinoic acid enhances the gene expression of human polymenic immunoglobulin receptor (pIgR) by TNF-α[J].Clin Exp Immunol,2004;135:448-454.
  • 5Liu D Y,Wang X L,Liu P.TNF-alfa upreglulates the expression of immunoglobulin secretory component[J].J Investing Allergol Clin Immunol,2007;17(2):101-106.
  • 6Murthy A K,Dubose C N,Banas J A et al.Contribution of polymeric immunoglobulin receptor to regulation of intestinal inflammation in dextran sulfate sodium-induced colitis[J].J Gastroenterol Hepatol,2006;21(9):1372-1380.
  • 7Perrier C,Sprenger N,Corthesy B.Glycans on secretory component participate in innate protection against mucosal pathogens[J].J Biol Chem,2006;281(20):14280-14287.
  • 8De Araujo A N,Giugliano L G.Lactoferrin and free secretory component of human milk inhibit the adhesion of enteropathogenic Escherichia coli to Hela cells[J].BMC Microbiol,2001;1(1):25-30.
  • 9Davids B J,Palm J E,Housley M P et al.Polymeric immunoglobulin receptor in intestinal immune defense against the lumen-dwelling protozoan parasite Giardia[J].J Immunol,2006;177(9):6281-6290.
  • 10Uren T K,Wijburg O L,Simmons C et al.Vaccine-induced protection against gastrointestinal bacterial infections in the absence of secretory antibodies[J].Eur J Immunol,2005;35(1):180-188.

引证文献3

二级引证文献11

中国免疫学杂志
2008年 第11期

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