摘要
目的:研究TNF-α对Caco-2细胞表达SC的影响。方法:采用免疫组化、ELISA、Western blot、Real-time PCR方法检测肠上皮细胞表达SC的变化。结果:与无TNF-α刺激比较,用不同浓度TNF-α刺激后SC阳性细胞、培养上清液中游离SC、Caco-2细胞SC蛋白和SC基因的表达明显增加(P<0.01)。结论:TNF-α上调Caco-2细胞内SC的表达。
Objective:To study the influence of TNF-α on the expression of secretory component (SC) in Caco-2 cells. Methods: Immunocytochemistry,ELISA, Western blot and quantitative real-time PCR were used to test SC-positive cells of Caco-2, free SC in culture super- natants, protein and mRNA expression of SC in the cells. Results: The increase of SC-positive cells, free SC in culture supematants, SC protein expression and expression of SC genes in Caco-2 cells was found under stimulation of TNF-α treatment compared with control( P 〈 0.01 ). Con. dusion: TNF-α upreglulats the expression of secretory component in Caco-2 cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2008年第11期993-996,共4页
Chinese Journal of Immunology
基金
国家自然基金资助项目(No.30670947)(No.30871158)
辽宁省教委课题资助项目(No.05L466)(No.2008783)