摘要
目的:研究中药复方制剂丹莪妇康胶囊(Danefukang capsule)对子宫内膜异位症(Endometriosis,EMs)大鼠模型在位、异位内膜组织中T细胞亚群及巨噬细胞数目的影响。方法:复制EMs大鼠模型,随机分为正常对照组、模型组、丹莪妇康胶囊低、中、高剂量组、阳性对照组,灌胃给药45天后,对在位、异位子宫内膜组织进行免疫组化染色,并用图像分析仪对其T细胞亚群、巨噬细胞定量分析。结果:与模型组相比,丹莪妇康胶囊中剂量组、高剂量组显著降低异位内膜组织内CD8+T细胞面数密度(P<0.05和P<0.01);中剂量组显著降低异位内膜组织内巨噬细胞数(P<0.05);虽然丹莪妇康胶囊对异位内膜组织内CD4+T细胞面数密度、CD8+T/CD4+T比值均有降低趋势,但未达统计学意义。结论:丹莪妇康胶囊能改善EMs大鼠异位内膜组织的免疫病理变化,主要是降低异位内膜组织内CD8+T细胞面数密度和巨噬细胞数目,可能是其疗效机制之一。
Objective： To study the effects of Danefukang capsule on T lymphocyte subpopulations and the number of macrophages in rats with endometriosis.Methods： The rats were induced by transplanting with endometrium surgically, and then were randomly divided into 6 groups：normal control group,EMs group,the treated EMs groups with Danefukang capsule （0.50,1.00,2.00 g/kg） and Danefukang soft extract （7.59 mg/kg）. After 45 days treatment, the density of CD8^＋ T cells and CD4^＋ T cells and the number of macrophages in endometrium and endometriotic tissue were determined by immunohistochemistry method. Results： Danefukang capsule signifieandy reduced the density of CD8^ T cells （ P 〈 0.01 or P 〈 0.05） and the number of macrophages in endometriotic tissue as compared to untreated-EMs rats （ P 〈 0.05）. Conclusion：The therapeutic effect of Danefukang capsule on rats with EMs is related to decreasing the density of CD8^＋ T cells and the number of macmphages in endometriotic tissue.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2008年第11期1007-1010,共4页
Chinese Journal of Immunology
基金
云南省科技厅新药研发专项基金资助项目(No.2004XY05)
