烟草中“多菌灵”残留量的液相色谱-质谱测定

Determination of Carbendazol Residue in Tobacco with LC/MS

用甲醇-盐酸溶液提取,固相萃取净化,反相HPLC/MS法测定了35个烤烟烟叶样品中的"多菌灵"残留量。结果表明:①方法的检测限为1.0μg/kg,回收率90.8～102.2,相对标准偏差2.05～4.21;②烟叶样品中多菌灵的检出率约为1/4,检出量5.7～112μg/kg。

The carbendazol residues in 35 tobacco samples were determined by LC/MS. Tobacco samples were extracted with methanol-HCl aqueous solution, then cleaned up with solid-phase extraction, and detected by reversed-phase LC/MS with XTerra MS C18 column, using aeetonitrile and 20 mmol/L ammonium acetate aqueous solution as a mobile phase at the flow rate of 0.2 mL/minute. The results showed that the limit of detection and average recoveries of carbendazol were 1.0 μg/kg and 90.8% to 102.2% , respectively, with the relative standard deviations （RSD） of 2.05% to 4.21%. The detection rate of carbendazol residues was about one fourth among the samples with the concentrations of 5.7 to 112 μg/kg.