A3α肽聚糖对凡纳滨对虾幼体酸性磷酸酶和碱性磷酸酶活力的影响

Effect of A3α Peptidoglycan on Acid Phosphatase and Alkaline Phosphatase Activities in Larval White Leg Shrimp Litopenaeus vannamei

用含不同质量分数（0.005%、0.01%、0.1%）A3α肽聚糖（PG）的饵料投喂及在育苗水体中添加不同质量浓度（0.005、0.01和0.05 mg/ml）的PG浸浴凡纳滨对虾幼体,以投喂不添加PG的饵料作对照,另设投喂活饵的试验组作参考;饲养7 d后,检测PL1期仔虾体内酸性磷酸酶（ACP）和碱性磷酸酶（ALP）活性。结果显示：PG经浸浴和口服能促进幼虾ACP和ALP活性提高;经活饵投喂的幼虾的ACP和ALP活性也较高。活饵组的ACP和ALP活性,0.01%PG添加量组和0.005 mg/ml PG的浸浴组幼体的ACP活性,0.01%和0.1%PG添加量组、PG质量浓度为0.01、0.05 mg/ml的浸浴组的ALP活性均较对照组有显著提高（P〈0.05）;口服组和浸浴组ACP活性均与PG浓度正相关,而口服组中0.01%PG添加量组ALP活性最高,浸浴组中PG浓度与ALP活性正相关。试验结果表明,PG能提高幼虾的非特异性免疫力。PG用于育苗的口服质量分数建议为0.05%-0.1%,浸浴质量浓度为0.05mg/ml。

Larval white leg shrimp Litopenaeus vannamei were fed steamed egg custard with 0.005%, 0.01% and 0.1% peptidoglycan（ PG）, and immersed in the seawater containing 0. 005, 0.01 and 0.05 mg/ml PG, and fed live food as control group. Acid phosphatase （ACP） and alkaline phosphatase （ALP） activities were tested after postlarvae （ PL1 ） were fed for 7 days. The activities of ACP and ALP were much higher in the postlarvae with oral administration and immersion than those in the control. There were much higher ACP and ALP activities in the larvae fed the live food, higher ACP activity in the larvae fed the food containing 0.01% PG and immersed in the seawater containing 0.05 mg/ml PG, higher ALP activity in the larvae fed the diet containing 0.01% and 0.1% PG and immersed in the seawater containing 0.01 mg/ml and 0.05 mg/ml PG higher than in the control larvae. For oral and immersion groups, the ACP activity was positively related to PG concentrations. The highest ALP activity was observed in the larvae fed the diet containing medium concentration of PG for the orally administrated groups. The ALP activity was positively related to the concentration of PG. An optimum concentration of 0.05% 0.1% for oral administration and 0.05 mg/ml for immersion were recommended in larvae culture.