血浆VEGF和PAI-1与糖尿病视网膜病变严重程度关系研究

The study of relationship between the levels of plasma VEGF,PAI-1 and the severity of the diabetic retinopathy

目的探讨不同程度糖尿病视网膜病变患者血浆中血管内皮生长因子(VEGF)和纤溶酶原激活物抑制物-1(PAT-1)水平的变化规律及其意义。方法收集2007年1月至12月新疆石河子大学医学院第一附属医院内分泌科和眼科就诊的2型糖尿病患者72例。用酶联免疫吸附双抗体夹心法检测血浆VEGF和PAT-1水平。结果(1)糖尿病患者的血浆VEGF质量浓度明显高于正常对照组(NC组)[(17.86±12.25)ng/L],背景期视网膜病变组(BDR组)[(93.41±54.69)ng/L]明显高于无视网膜病变组(NDR组)[(52.17±21.81)ng/L]和增殖期视网膜病变组(PDR组)[(61.24±37.55)ng/L],但PDR组与NDR组比较差异无统计学意义;(2)糖尿病患者血浆PAI-1高于正常对照组,BDR组[(58.29±20.53)μg/L]和PDR组[(66.84±23.81)μg/L]明显高于NDR组[(44.88±16.36)μg/L],但BDR组和PDR组比较差异无统计学意义。结论糖尿病视网膜病变患者存在着血管内皮细胞的损伤和低纤溶状态,检测糖尿病患者血浆中VEGF和PAI-1对糖尿病视网膜病变的早期诊断和及时干预治疗可能具有重要意义。

Objective To observe the relationship between the levels of plasma VEGF,PAI-1 and the severity of the diabetic retinopathy in type 2 diabetic patients. Methods ELISA method was used to measure the levels of VEGF and PAI-1. Results The level of VEGF was increased in all diabetic patients compared with the normal eontrol group[ （ 17.86 ± 12. 25）ng/L] ,and that of the patients with BDR[ （93.41 ±54. 69）ng/L]was higher than that of the patients with NDR [ （52. 17 ± 21.81 ）ng/L] and PDR [ （61.24 ± 37.55 ）ng/L ]. The level of PAI-1 was also increased in all diabetic patients. The increase tendency was more obvious in patients with BDR [ （ 58.29 ± 20. 53 ） μg/L ] and PDR [ （ 66. 84 ± 23.81 ） μg/L]. Conclusion The damage of the vascular endothelial cell and the impaired fibrinolysis state exist in diabetic retinopathy. So it is very important to measure the levels of VEGF and PAI-1 for the early diagnosis and intervention treatment of diabetic retinopathy.