60株快速生长分枝杆菌对11种抗结核化疗药物的敏感性分析

Sensitivity of 60 Rapid Growing Mycobacteria to Various Anti-Tuberculosis Drugs

目的了解脓肿、龟、偶发3种快速生长分枝杆菌对11种抗结核化疗药物的敏感性。方法应用绝对浓度法对脓肿、龟、偶发分枝杆菌进行药物敏感性测定,测定药物及其浓度(μg/mL)分别为:异烟肼(1、10)、利福平(50、250)、链霉素(10、100)、乙胺丁醇(5、50)、阿米卡星(10、100)、力克肺疾(1、10)、卷曲霉素(100、1000)、环丙沙星(1、10)、莫西沙星(1、10)、左氧氟沙星(1、10)和克拉霉素(10、100)。结果共检测31株脓肿、20株龟和9株偶发分枝杆菌菌株。敏感性高于10的有:脓肿分枝杆菌对克拉霉素87.1、脓肿分枝杆菌对阿米卡星22.6、龟分枝杆菌对克拉霉素75.0、龟分枝杆菌对阿米卡星55.0、偶发分枝杆菌对克拉霉素77.8、偶发分枝杆菌对阿米卡星33.3。另外,3种分枝杆菌虽对乙胺丁醇、链霉素、环丙沙星低浓度敏感性低,但对其高浓度则表现相当程度的敏感性。结论脓肿、龟、偶发3种快速生长杆菌对克拉霉素具有较高的敏感性。

Objective To evaluate the sensitivity of rapid growing Mycobacterium, including M. abscessus, M. chelonae and M. fortuitum, to various anti-tuberculosis drugs. Methods The sensitivity of mycobacteria to antituberculosis drugs was evaluated using the Absolute Concentrate Method. 11 anti-tuberculosis drugs were used in this study. Two different concentrations （ug/mL） were used for each drug. These included Isoniazid （1/10）, Rifampin （50/250）, Streptomyain （10/100）, Ethambutol（5/50）, Amikacin（10/100）, Pasiniazid （1/10）, Capreomycin （100/1000）, Ciprofloxacin （1/10）, Moxifloxacin （1/10）, Lcvofloxacin （1/10） and Clarithromycin （1/10）. Results A total of 31 strains of M. abscessus, 20 strains of M. chelonoe and 9 strains of M. fortuitum were studied. The sensitivity of M. abscessus, M. chelonae and M. fortuitum to clarithromycin were 87.1%, 75.0% and 77.8%, respectively. The sensitivity of M. abscessus, M. chelonae and M. fortuitum to Amikacin were 22.6%, 55.0% and 33.3%, respectively. Moreover, these 3 species of mycobacteria were found to be sensitive to high, but not low, concentration of Ethambutol, Streptomycin and Ciprofloxecin. Conclusion All three species of M. abscessus, M. chelonae and M. fortuitum were found to be sensitive to clarithromycin.