星形胶质细胞通过激活PKA信号通路在神经干细胞增殖、分化中的作用

Role of astrocyte in the proligeration and differentiation of nerve stem cells by activating PKA signal pathway

目的:研究星形胶质细胞(Astrocyte,AS)对PKA信号通路的激活在神经干细胞(Neural stem cell,NSC)增殖、分化中的调节作用。方法:取孕13d胎鼠大脑皮层分散培养NSC,应用AS培养上清刺激NSC,以及应用PKA受体拮抗剂H89预处理大脑皮层NSC后,用Westernblot方法测定NSC内PKA、nestin、NSF的含量变化,采用MTT测量和分析AS对培养的NSC增殖和定向分化的影响。结果:正常对照组的NSCNSF含量较低,AS刺激后,nestin含量迅速降低,而NSF的含量则逐渐升高;H89(15μmol/L)可明显抑制AS刺激NSC后NSF含量的增加。结论:AS可通过激活NSCPKA信号通路,促进NSC增殖和分化。

Objective：To study the effect of astrocyte activating PKA signal pathway on the proligeration and differentiation of nerve stem cells.Methods：Cerebral cortex of 13-day embryo mice was taken and the nerve stem cells were cultured scatteringly. Astrocyte was used to stimulate the nerve stem cells,and H89, PKA recepter antagonist, was used to pretreat the nerve stem cells of the cerebral cortex, then the contents of PKA,nestin,and NSF in the nerve stem cells were determined using western blot. MTT was applied to determine and analyse the effect of horizontal cell on the proliferation and orientational differentiation of cultured nerve stem cells. Results：In the normal control group the content of NSF in the nerve stem cells was low; after astrocyte stimulation the nestin content decreased rapidly,while the content of NSF increased gradually. Hag（15 μmol/L） could markedly supress the increase of NSF content in the nerve stem cell stimulated by horizontal cells. Conclusion：Astrocyte can promote the proliferation and differentiation of nerve stem cells by activating PKA signal pathway of nerve stem cells.