藏花素对膀胱移行细胞癌的增殖抑制作用

Proliferation inhibitory effect of crocin on transitional cell carcinoma of urinary bladder

目的:研究藏花素(Crocin)对膀胱移行细胞癌是否具有增殖抑制作用。方法:MTT法检测藏花素对T24细胞增殖的影响;应用形态学手段观察细胞形态学的改变;流式细胞术检测肿瘤细胞周期动力学。荷瘤小鼠模型建立,测定藏花素处理后荷瘤小鼠体内抑瘤率。结果:藏花素能明显抑制T24细胞的增殖,并可导致其形态学的改变;藏花素作用后G0/G1期细胞比例逐渐增加,S期和G2/M期细胞比例逐渐减少;对照组裸鼠肿瘤的平均体积明显大于藏花素处理组裸鼠肿瘤的平均体积,其差异具有显著性(P<0.05),裸鼠生长抑制率为27.8%。结论:体内外实验证明,藏花素对膀胱移行细胞癌的增殖有抑制作用。

Objective：To investigate the proliferation inhibitory effect of crocin on transitional cell carcinoma of urinary bladder（TCCB）. Methods：MTr assay was used to evaluate the inhibitory effect of Crocin on the proliferation of T24 cells. Microscope was used to observe the morphological changes of the T24 cells. Flow cytometry was used to measure the cell cycle. T24 cells were inoculated in to BALB/c nude mice to establish bladder cancer model. After treatment with Crocin,the inhibitory effects were observed on the growth of tumors. Results：The proliferation of T24 cells were inhibited remarkably. Morphological changes of cells were observed. Flow cytometric profiles revealed that crocin led to the increase of the cells in G0/G1 phase and the decrease of cells in S phase and G2/M（P〈0.05）,Crocin could inhibit the growth of BALB/c xenograft tumor significant r. The inhibitory rate of tumor was 28.7%. Conclusion：Crocin exerts an inhibitory effect on the growth of transitional cell carcinoma of urinary bladder in vitro and vivo.