系统性红斑狼疮患者血清骨桥蛋白表达的变化及其临床意义

The expressed changes and clinical significance of serum osteopontin in systemic lupus erythematosus patients

研究骨桥蛋白(Osteopontin,OPN)与狼疮性肾炎(Lupus nephritis,LN)的相关性,探讨系统性红斑狼疮(Systemic lupus erythematosus,SLE)患者血清OPN表达的变化在LN诊断中的价值。方法:用酶联免疫吸附(Sandwichenzyme-linkedim-munosorbentassay,ELISA)法检测68例SLE患者(其中合并LN47例、无肾炎表现的SLE患者21例)及20名正常对照组血清中OPN的水平。结果:68例SLE患者中血清OPN阳性率79.41%,20名正常对照组OPN均阴性,SLE患者血清OPN阳性率及OPN水平显著高于正常对照组(P<0.01),也明显高于抗dsDNA抗体的阳性率(55.88%),差异具有显著性(P<0.01);LN患者组与无肾炎表现的SLE患者组比较,血清OPN阳性率差异具有显著性(P<0.05);LN组患者血清OPN水平高于无肾炎表现的SLE组,差异具有显著性(P<0.05);LN组患者的血清OPN阳性率高于抗dsDNA抗体阳性率,差异具有显著性(P<0.05);LN患者血清OPN水平与24h尿蛋白定量的高低呈明显的正相关(r=0.313;P<0.05)。结论:OPN可能与LN的发生、发展有关,可作为诊断LN的一种疾病指标。

Objective：To research the value of expressed changes in serum osteopontin（OPN） of systemic lupus erythematosus （SLE） patients in the diagnosis of lupus nephritis（LN） by studying the correlation between OPN and LN. Methods：The enzyme-linked immunosorbent assay（ELISA） was used to mensurate the level of OPN in the sera of 47 LN,21 SLE patients without clinical symptoms of nephritis,and 20 healthy controls. Results：The positive ratio of serum OPN was 79.41% in 68 SLE patients and was 0% in healthy controls.Compared with healthy controls,the positive ratios and level of serum OPN in SLE patients were significantly higher（P〈0.01 ）. The positive ratio of serum OPN in SLE patients was significantly higher than that of anti-dsDNA antibody and anti-Sin antibody（P〈0.01）.There were significant differences in the positive ratios and level of serum OPN between LN and SLE without clinical symptoms of nephritis（P〈0.05）, respectively. The positive ratio of serum OPN in LN patients was higher than that of anti-dsDNA antibody （P〈0.05 ）.The level of serum OPN in LN patients was correlated with the fixed quantity of urine protein for 24 hours（r=0.313;P〈0.05）. Conclusion：OPN may be related with the onset and development of LN,which may serve as a disease marker of LN.