摘要
目的:制备具有杀伤活性的新型抗白血病免疫毒素。方法:在异丙基硫代-β-D-半乳糖苷(Isopropyl-beta-D-throgalac-top yranoside,IPTG)的诱导下,高效表达免疫毒素IL3-PE38KDEL,采用细胞毒性实验,对毒素活性进行检测。结果:免疫毒素IL3-PE38KDEL以包涵体形式为主高效表达,该蛋白具有较好的毒素活性。结论:免疫毒素IL3-PE38KDEL具有良好的生物学活性,为其作为抗白血病药物的进一步研究打下了基础。
Objective：To prepare a new type of anti-leukemia immunotoxin with killing activity Methods：The method of cytotoxicity was used to study the activity of the immunotoxin after the induction of IPTG. Results ：The expressed fusion proteins were detected mostly as inclusion bodies at high level.The result showed IL3-PE38KDEL had liable activity of toxicity. Conclusion： The fusion protein IL3-PE38KDEL has good biological activity,which paves way for the further study on its treatment of leukemia.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第11期1362-1364,共3页
Journal of Chongqing Medical University
关键词
白介素3
假单胞菌外毒素
免疫毒素
Human interleukin-3
Pseudomonas exotoxin（PE38KDEL）
Immunotoxin
