应用高效液相色谱-蒸发光散射检测法测定大蒜中糖含量

Determination of Carbohydrates in Allium sativum L. by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

[目的]建立简便、准确、灵敏的测定大蒜中果糖、葡萄糖及蔗糖含量的方法。[方法]样品经热水提取后，使用Kromasil NH2（250mm×4．6mm，5μm）柱分离，以乙腈-水（80：20，V／V）为流动相，使用蒸发光散射检测器检测。[结果]果糖、葡萄糖和蔗糖的线性范围分别为0．05～3、0．075-5、0．05～5g/L；检出限分别为1．9、2．4、1．8μg/L；样品的平均加标回收率分别为99．2％、97．6％、98．0％；相对标准偏差分别为1．02％、1．41％、1．27％。[结论]该方法操作简便、快速、准确，用于实际样品的测定，结果令人满意。

[ Objective ] The aim was to develop a simple, accurate and sensitive method for determination of carbohydrates in Allium sativum L. [ Method] The carbohydrates in Allium sativum L. were extracted with hot water and separated on Kromasil NH2 column（250 mm× 4.6 mm, 5μm） using acetonitrile and water as the mobile phase with volume ratio of 80：20. The elute from the column was examined by an evaporative light scattering detector. [ Result ] The linear ranges of fructose, glucose and sucrose were 0.05 - 3, 0.075 - 5 and 0.05 - 5 g/L. The detection limits were 1.9, 2.4, and 1.8 μg/L. The average recovery were 99.2%, 97.6% and 98.0%, and the relative standard deviation were 1.02%, 1.41% and 1.27%, respectively. [ Conclusion] The method was applied to the determination of carbohydrates in Allium sativum L. with satisfactory results and it was found to be simple,fast and accurate.