草原龙胆无性快繁体系建立的初探

Preliminary Research on the Establishment of Multiplication System of Rough Gentian

[目的]研究草原龙胆以茎段为外植体的丛生芽的诱导与增殖情况。[方法]以草原龙胆为试材,用生长健壮的草原龙胆植株茎段为外植体建立其高效快繁体系,以MS、MB、N63种培养基及IBA,6-BA,GA 3种激素进行正交试验设计。[结果]结果表明,适宜的脱毒材料为0.1%的HgC l2。当基本培养基为MB(MS大量+B5微量+B5有机+Fe盐),IBA为2.0 mg/L,6-BA为0.5mg/L,GA为2.0 mg/L时,其丛生芽的诱导率、分蘖率最高。[结论]该试验结果可用于组织培养快速繁殖草原龙胆。

[Objective] The bud induction and its multiplication of rough gentian were researched in the experiment. [Method] The healthy rough gentian stem being taken as explants, the high-efficiency multiplication system was set up through the orthogonal design including 3 media（MS, MB and N6） and 3 kinds of plant growth regulators（IBA ,6-BA and GA）. [Results] The results showed that 0.1% HgCl2 was the best sterilized agent. When the fundamental medium was the MB（ macro-element of MS ＋ trace element and organic material of B5＋Fe） and IBA was 2.0 mg/L, 6-BA was 0.5 mg/L, GA was 2.0 mg,/L, the rate of shoot induction and multiplication of rough gentian were highest. [Conclusion] The results could be used in tissue culture and rapid multiplication of rough gentian.