南方紫花苜蓿及其原生质体再生植株同工酶活性的对比研究

Comparative Study on Isozyme Activity of Alfalfa in Southern China and Its Protoplast-derived Plants

[目的]为南方紫花苜蓿的生物技术研究提供依据。[方法]以适宜我国南方环境的XN-1型(西农1号)紫花苜蓿为材料,测定其母系植株及原生质体再生植株茎、叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)3种同工酶活性,并进行对比分析。[结果]在同样的胁迫条件下,南方紫花苜蓿母系植株的3种同工酶活性均高于原生质体再生植株;母系植株与原生质体再生植株过氧化物酶活性存在极显著差异(P<0.01),超氧化物歧化酶、过氧化氢酶活性存在显著差异(P<0.05)。[结论]南方紫花苜蓿原生质体再生植株的3种同工酶活性较母系植株均有所下降,相应的抗性、适应性也较母系植株下降。

[Objective] The study was to provide the basis for biotechnology research on alfalfa in Southern China. [ Method ] With XN-1 type （Xinong 1） alfalfa suitable to environment of Southern China as materials, the activities of 3 isozymes such as superoxide dismntase （SOD） ,catalase （CAT） and peroxidase （POD） in stem and leaf of matrilineal plants and protoplast-derived plants of alfalfa were detected, and the comparative analysis on their isozyme activities was conducted. [Result] Under the same stress condition, the activities of 3 isozymes of matrilineal plants of alfalfa in Southern China were all higher than that of its potoplast-derived plants. The POD activity of matrilineal plants had extremely significant difference（P 〈0.01）with that of potoplast-derived plants, and the activities of SOD and CAT between two kinds of plants also had significant difference（ P 〈 0.05 ）. [Conclusion] The activities of 3 isozymes of protoplast-derived plants of alfalfa in Southern China all had some decrease than that of matrilineal plants, and their relevant resistance, adaptability were also decreased than that of matri- lineal plants.